• Product name

    Anti-Aprataxin antibody [EPR8751]
    See all Aprataxin primary antibodies
  • Description

    Rabbit monoclonal [EPR8751] to Aprataxin
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Aprataxin aa 100-200. The exact sequence is proprietary.
    Database link: Q7Z2E3

  • Positive control

    • Human cerebellum and Human fetal brain lysates; HeLa, A549 and 293 cell lysates. Human cervix carcinoma tissue. HeLa cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab192598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.


  • Function

    DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity.
  • Tissue specificity

    Widely expressed. In brain, it is expressed in the posterior cortex, cerebellum, hippocampus and olfactory bulb. Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2.
  • Involvement in disease

    Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA) [MIM:208920]. AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy.
    Defects in APTX are a cause of coenzyme Q10 deficiency (COQ10D) [MIM:607426]. Coenzyme Q10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy.
  • Sequence similarities

    Contains 1 C2H2-type zinc finger.
    Contains 1 FHA-like domain.
    Contains 1 HIT domain.
  • Domain

    The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
    The FHA-like domain mediates interaction with NCL; XRCC1 and XRCC4.
    The HIT domain is required for enzymatic activity.
    The C2H2-type zinc finger mediates DNA-binding.
  • Cellular localization

    Nucleus > nucleoplasm. Nucleus > nucleolus. Upon genotoxic stress, colocalizes with XRCC1 at sites of DNA damage. Colocalizes with MDC1 at sites of DNA double-strand breaks. Interaction with NCL is required for nucleolar localization.
  • Information by UniProt
  • Database links

  • Alternative names

    • AOA 1 antibody
    • AOA antibody
    • AOA1 antibody
    • Aprataxin antibody
    • Aprataxin homolog antibody
    • Aptx antibody
    • APTX_HUMAN antibody
    • Ataxia 1 early onset with hypoalbuminemia antibody
    • Ataxia1 early onset with hypoalbuminemia antibody
    • AXA 1 antibody
    • AXA1 antibody
    • EAOH antibody
    • EOAHA antibody
    • FHA HIT antibody
    • FHA-HIT antibody
    • FLJ20157 antibody
    • Forkhead associated domain histidine triad like antibody
    • Forkhead associated domain histidine triad like protein antibody
    • Forkhead-associated domain histidine triad-like protein antibody
    • MGC1072 antibody
    see all


  • All lanes : Anti-Aprataxin antibody [EPR8751] (ab192598) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : Hman cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Anti-Aprataxin antibody [EPR8751] (ab192598) at 1/2000 dilution + Human fetal brain lysate at 10 µg

    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 29-40 kDa (possible isoform)

    Identify seven isoforms

  • Immunohistochemical analysis of paraffin-embedded, Human cervix carcinoma tissue labeling Aprataxin with ab192598 at a 1/1000 dilution. Counter stained with hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling Aprataxin with ab192598 at a 1/200 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.


This product has been referenced in:

  • Horton JK  et al. XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity. DNA Repair (Amst) 64:26-33 (2018). Read more (PubMed: 29477978) »
See 1 Publication for this product

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