Key features and details
- Rabbit polyclonal to APRIL/TNFSF13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-APRIL/TNFSF13 antibody
See all APRIL/TNFSF13 primary antibodies
DescriptionRabbit polyclonal to APRIL/TNFSF13
This antibody detects endogenous levels of total APRIL/TNFSF13 protein.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human brain cortex tissue; HeLa and COLO205 cell lysates; HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 27 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionCytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Tissue specificityExpressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe precursor is cleaved by furin.
- Information by UniProt
- A proliferation inducing ligand antibody
- A proliferation-inducing ligand antibody
- APRIL antibody
Immunohistochemical analysis of paraffin embedded formalin fixed human brain cortex tissue labeling APRIL/TNFSF13 using ab189263 at 10 µg/ml.
Immunofluorescence of HeLa cells labeling APRIL/TNFSF13 using ab189263 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.
All lanes : Anti-APRIL/TNFSF13 antibody (ab189263) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : COLO205 cell lysate
Lane 3 : COLO205 cell lysate with immunizing peptide
Predicted band size: 27 kDa
ab189263 has not yet been referenced specifically in any publications.