Recombinant
RabMAb

Recombinant Anti-APRIL/TNFSF13 antibody [EPR4286] (ab108206)

Overview

  • Product name

    Anti-APRIL/TNFSF13 antibody [EPR4286]
    See all APRIL/TNFSF13 primary antibodies
  • Description

    Rabbit monoclonal [EPR4286] to APRIL/TNFSF13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human APRIL/TNFSF13 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Raji, Jurkat, HeLa, PC-12 or NIH 3T3 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4286
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
ICC 1/100 - 1/250.

Target

  • Function

    Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • Tissue specificity

    Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The precursor is cleaved by furin.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A proliferation inducing ligand antibody
    • A proliferation-inducing ligand antibody
    • APRIL antibody
    • CD256 antibody
    • TALL-2 antibody
    • TALL2 antibody
    • TNF related death ligand antibody
    • TNF- and APOL-related leukocyte expressed ligand 2 antibody
    • TNF-related death ligand 1 antibody
    • TNF13_HUMAN antibody
    • TNFSF13 antibody
    • TRDL-1 antibody
    • TRDL1 antibody
    • Tumor necrosis factor (ligand) superfamily, member 13 antibody
    • Tumor necrosis factor ligand superfamily member 13 antibody
    • Tumor necrosis factor like protein ZTNF2 antibody
    • Tumor necrosis factor related death ligand 1 antibody
    • UNQ383/PRO715 antibody
    • ZTNF2 antibody
    see all

Images

  • All lanes : Anti-APRIL/TNFSF13 antibody [EPR4286] (ab108206) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 27 kDa

References

ab108206 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cell (Brain tissue)
Specification
Brain tissue
Permeabilization
Yes - 0.3% Triton 100x
Fixative
Paraformaldehyde

Shuxian Hu

Verified customer

Submitted Nov 20 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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