Overview

  • Product name

  • Description

    Rabbit polyclonal to APRT
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total APRT protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human APRT.
    Database link: P07741

  • Positive control

    • MCF7, 22RV-1, THP1, A549, K562, HeLa and mouse intestine lysates. Human thyroid cancer tissue. A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.

Target

  • Function

    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • Pathway

    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • Involvement in disease

    Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • Sequence similarities

    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

Images

  • All lanes : Anti-APRT antibody (ab196558) at 1/500 dilution

    Lane 1 : MCF7 extract
    Lane 2 : 22RV-1 extract
    Lane 3 : THP1 extract
    Lane 4 : A549 extract
    Lane 5 : K562 extract
    Lane 6 : HeLa extract
    Lane 7 : Mouse intestine extract

    Predicted band size: 20 kDa

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling APRT with ab196558 at 1/200 dilution.

  • Immunofluorescence analysis of A549 cells labeling APRT using ab196558, at 1/50 dilution

References

ab196558 has not yet been referenced specifically in any publications.

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