Key features and details
- Rabbit polyclonal to APRT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-APRT antibody
See all APRT primary antibodies
DescriptionRabbit polyclonal to APRT
SpecificityThis antibody detects endogenous level of total APRT protein.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Recombinant full length protein corresponding to Human APRT.
Database link: P07741
- MCF7, 22RV-1, THP1, A549, K562, HeLa and mouse intestine lysates. Human thyroid cancer tissue. A549 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 20 kDa.|
FunctionCatalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
PathwayPurine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
Involvement in diseaseDefects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
Sequence similaritiesBelongs to the purine/pyrimidine phosphoribosyltransferase family.
- Information by UniProt
- Adenine phosphoribosyltransferase antibody
- AMP antibody
- AMP diphosphorylase antibody
All lanes : Anti-APRT antibody (ab196558) at 1/500 dilution
Lane 1 : MCF7 extract
Lane 2 : 22RV-1 extract
Lane 3 : THP1 extract
Lane 4 : A549 extract
Lane 5 : K562 extract
Lane 6 : HeLa extract
Lane 7 : Mouse intestine extract
Predicted band size: 20 kDa
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling APRT with ab196558 at 1/200 dilution.
Immunofluorescence analysis of A549 cells labeling APRT using ab196558, at 1/50 dilution
ab196558 has been referenced in 1 publication.
- Huyet J et al. Structural Insights into the Forward and Reverse Enzymatic Reactions in Human Adenine Phosphoribosyltransferase. Cell Chem Biol 25:666-676.e4 (2018). PubMed: 29576532