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Rabbit polyclonal to APRT
Predicted to work with:
Recombinant protein fragment corresponding to a region within amino acids 1 and 104 of APRT.
WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate;
ICC/IF: HeLa cells;
IHC-P: DLD-1 xenograft.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 20 kDa.
1/100 - 1/500.
1/100 - 1/200.
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
Involvement in disease
Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Information by UniProt
Adenine phosphoribosyltransferase antibody
AMP diphosphorylase antibody
Western blot - Anti-APRT antibody (ab96025)
Anti-APRT antibody (ab96025) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 20 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-APRT antibody (ab96025)
Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96025 at 1/200 dilution.
Bottom panel: merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APRT antibody (ab96025)
ab96025, at 1/500 dilution, staining APRT in formalin-fixed, paraffin-embedded DLD-1 xenograft by immunohistochemistry.
has not yet been referenced specifically in any publications.
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