Overview

  • Product name

    Anti-APX2 antibody (HRP)
    See all APX2 primary antibodies
  • Description

    Rabbit polyclonal to APX2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Arabidopsis thaliana
  • Immunogen

    Recombinant full length protein corresponding to Arabidopsis thaliana APX2 aa 4-250.
    Database link: Q1PER6

  • General notes

     This product was previously labelled as L-ascorbate peroxidase 2, L-ascorbate peroxidase 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab192968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 28 kDa.

Target

  • Function

    Plays a key role in hydrogen peroxide removal.
  • Tissue specificity

    Detected in bundle sheath cells, the photosynthetic cells that surround the phloem and xylem.
  • Sequence similarities

    Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

    • Alternative names

      • APX1b antibody
      • APX2 antibody
      • APX2_ARATH antibody
      • AtAPx02 antibody
      • cytosolic antibody
      • L-ascorbate peroxidase 1b antibody
      • L-ascorbate peroxidase 2 antibody
      see all

    Images

    • Western blot analysis of Arabidopsis thaliana lysate at 80μg, 40μg and 20μg labeling L-ascorbate peroxidase 2 with ab192968 at 0.6µg/ml.

    References

    ab192968 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Soybean Cell lysate - whole cell (HeLa cells transfected with mutated soybean APX)
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Loading amount
    50000 cells
    Specification
    HeLa cells transfected with mutated soybean APX
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Feb 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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