Anti-AQP-6 antibody - C-terminal (ab191061)
Key features and details
- Rabbit polyclonal to AQP-6 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AQP-6 antibody - C-terminal -
Description
Rabbit polyclonal to AQP-6 - C-terminal -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AQP-6 aa 264-282 (C terminal).
Sequence:EPLKKESQPGSGAVEMESV
Database link: Q13520 -
Positive control
- U87, COLO320 and MCF7 cell lysates. Human placenta and human lung cancer tissue.
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General notes
Previously labelled as Aquaporin 6.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab191061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Forms a water-specific channel that participates in distinct physiological functions such as glomerular filtration, tubular endocytosis and acid-base metabolism. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Cytoplasmic vesicle membrane. - Information by UniProt
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Database links
- Entrez Gene: 363 Human
- Omim: 601383 Human
- SwissProt: Q13520 Human
- Unigene: 54505 Human
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Alternative names
- AQP-6 antibody
- AQP2L antibody
- Aqp6 antibody
see all
Images
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Western blot - Anti-AQP-6 antibody - C-terminal (ab191061)All lanes : Anti-AQP-6 antibody - C-terminal (ab191061) at 0.5 µg/ml
Lane 1 : U87 cell lysate
Lane 2 : COLO320 cell lysate
Lane 3 : MCF7 cell lysate
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling AQP-6 with ab191061 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling AQP-6 with ab191061 at 1 μg/ml.
Protocols
References (1)
ab191061 has been referenced in 1 publication.
- Michalek K & Grabowska M Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle. Res Vet Sci 125:162-169 (2019). PubMed: 31233960
