Anti-AQP-6 antibody - C-terminal (ab191061)

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Overview

  • Product name

    Anti-AQP-6 antibody - C-terminal

  • Description

    Rabbit polyclonal to AQP-6 - C-terminal

  • Host species

    Rabbit

  • Specificity

    No cross reactivity with other proteins.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human AQP-6 aa 264-282 (C terminal).
    Sequence:

    EPLKKESQPGSGAVEMESV


    Database link: Q13520

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • U87, COLO320 and MCF7 cell lysates. Human placenta and human lung cancer tissue.

  • General notes

    Previously labelled as Aquaporin 6.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Forms a water-specific channel that participates in distinct physiological functions such as glomerular filtration, tubular endocytosis and acid-base metabolism.

  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.

  • Domain

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

  • Cellular localization

    Cytoplasmic vesicle membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AQP-6 antibody
      • AQP2L antibody
      • Aqp6 antibody
      • AQP6_HUMAN antibody
      • Aquaporin 2 like antibody
      • aquaporin 2 like kidney specific antibody
      • Aquaporin 6 antibody
      • Aquaporin 6 kidney specific antibody
      • Aquaporin-2-like antibody
      • Aquaporin-6 antibody
      • hKID antibody
      • Human kidney water channel (hKID) mRNA, complete cds antibody
      • KID antibody
      • Kidney-specific aquaporin antibody
      see all

    Images

    • Western blot - Anti-AQP-6 antibody - C-terminal (ab191061)
      Western blot - Anti-AQP-6 antibody - C-terminal (ab191061)
      All lanes : Anti-AQP-6 antibody - C-terminal (ab191061) at 0.5 µg/ml

      Lane 1 : U87 cell lysate
      Lane 2 : COLO320 cell lysate
      Lane 3 : MCF7 cell lysate

      Predicted band size: 29 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling AQP-6 with ab191061 at 1 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP-6 antibody - C-terminal (ab191061)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue labeling AQP-6 with ab191061 at 1 μg/ml.

    References

    This product has been referenced in:

    • Michalek K & Grabowska M Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle. Res Vet Sci 125:162-169 (2019). Read more (PubMed: 31233960) »
    See 1 Publication for this product

    Publishing research using ab191061? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AQP-6 antibody - Conformation-Specific

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (intervertebral disc)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 1X TBS, 0.1% calcium chloride/alpha-chymotrypsin
    Permeabilization
    No
    Specification
    intervertebral disc
    Blocking step
    1% BSA in 75% TBS 35% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
    Fixative
    10% neutral buffered formalin
    Read More

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    Verified customer

    Submitted Jun 05 2017

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