Overview

  • Product name

  • Description

    Rabbit polyclonal to AQP0
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of AQP0.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human AQP0 (internal sequence).

  • Positive control

    • HT29 cell extract; Human brain tissue
  • General notes

    Previously labelled as Aquaporin 0. 

Properties

Applications

Our Abpromise guarantee covers the use of ab134695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Water channel. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.
  • Tissue specificity

    Major component of lens fiber gap junctions.
  • Involvement in disease

    Cataract 15, multiple types
  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain

    Aquaporins contain two tandem repeats each containing two membrane-spanning helices and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). Each tandem repeat contains a loop and a short helix that enter and leave the lipid bilayer on the same side.
  • Post-translational
    modifications

    Subject to partial proteolytic cleavage in the eye lens core. Partial proteolysis promotes interactions between tetramers from adjoining membranes.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • AQP 0 antibody
    • AQP0 antibody
    • Aquaporin antibody
    • Aquaporin 0 antibody
    • Aquaporin-0 antibody
    • Aquaporin0 antibody
    • Lens fiber major intrinsic protein antibody
    • LIM 1 antibody
    • LIM1 antibody
    • Major intrinsic protein 26kD antibody
    • Major intrinsic protein of lens fiber antibody
    • MIP 26 antibody
    • mip antibody
    • MIP_HUMAN antibody
    • MIP26 antibody
    • MP 26 antibody
    • MP26 antibody
    • PALM antibody
    see all

Images

  • Anti-AQP0 antibody (ab134695) at 1/500 dilution + HT29 cell extract at 30 µg

    Predicted band size: 28 kDa

  • Immunohistochemical analysis of AQP0 in paraffin-embedded Human brain tissue using ab134695 at 1/50 dilution.

References

ab134695 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab134695.
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