Overview

  • Product name

    Anti-AQP0 antibody - C-terminal
    See all AQP0 primary antibodies
  • Description

    Rabbit polyclonal to AQP0 - C-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse AQP0 aa 246-263 (C terminal).
    Sequence:

    NGQPEGTGEPVELKTQAL


    Database link: P51180

  • General notes

    Previously labelled as Aquaporin 0. 

Properties

Applications

Our Abpromise guarantee covers the use of ab191020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • Function

    Water channel. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.
  • Tissue specificity

    Major component of lens fiber gap junctions.
  • Involvement in disease

    Cataract 15, multiple types
  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain

    Aquaporins contain two tandem repeats each containing two membrane-spanning helices and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). Each tandem repeat contains a loop and a short helix that enter and leave the lipid bilayer on the same side.
  • Post-translational
    modifications

    Subject to partial proteolytic cleavage in the eye lens core. Partial proteolysis promotes interactions between tetramers from adjoining membranes.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • AQP 0 antibody
    • AQP0 antibody
    • Aquaporin antibody
    • Aquaporin 0 antibody
    • Aquaporin-0 antibody
    • Aquaporin0 antibody
    • Lens fiber major intrinsic protein antibody
    • LIM 1 antibody
    • LIM1 antibody
    • Major intrinsic protein 26kD antibody
    • Major intrinsic protein of lens fiber antibody
    • MIP 26 antibody
    • mip antibody
    • MIP_HUMAN antibody
    • MIP26 antibody
    • MP 26 antibody
    • MP26 antibody
    • PALM antibody
    see all

References

ab191020 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Intervertebral Disc)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Intervertebral Disc
Blocking step
1% BSA in 75% TBS and 25% Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
Fixative
10% Neutral Buffered Formalin

Abcam user community

Verified customer

Submitted Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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