Recombinant Anti-AQP8 antibody [EPR8397] (ab133667)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8397] to AQP8
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-AQP8 antibody [EPR8397]
See all AQP8 primary antibodies -
Description
Rabbit monoclonal [EPR8397] to AQP8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
corresponding to Human AQP8 aa 100-200 (extracellular).
Database link: O94778 -
Positive control
- HeLa, BxPC-3 and Caco-2 cell lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Aquaporin 8
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.60 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8397 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. -
Tissue specificity
Expressed only in pancreas and colon. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 343 Human
- Omim: 603750 Human
- SwissProt: O94778 Human
- Unigene: 176658 Human
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Alternative names
- AQP 8 antibody
- AQP-8 antibody
- AQP8 antibody
see all
Images
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All lanes : Anti-AQP8 antibody [EPR8397] (ab133667) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa -
Flow cytometry analysis of HeLa cells labelling AQP8 with ab133667 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133667 has been referenced in 1 publication.
- Pellavio G et al. HPV Infection Affects Human Sperm Functionality by Inhibition of Aquaporin-8. Cells 9:N/A (2020). PubMed: 32429588