RecombinantRabMAb

Recombinant Anti-AQP8 antibody [EPR8397] (ab133667)

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Western blot - Anti-AQP8 antibody [EPR8397] (ab133667)
  • Flow Cytometry - Anti-AQP8 antibody [EPR8397] (ab133667)
  • OI-RD Scanning - Anti-AQP8 antibody [EPR8397] (ab133667)
  • Anti-AQP8 antibody [EPR8397] (ab133667)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8397] to AQP8
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-AQP8 antibody [EPR8397]
    See all AQP8 primary antibodies

  • Description

    Rabbit monoclonal [EPR8397] to AQP8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    corresponding to Human AQP8 aa 100-200 (extracellular).
    Database link: O94778

  • Positive control

    • HeLa, BxPC-3 and Caco-2 cell lysates; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Aquaporin 8

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 4.60 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8397

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.

    • Tissue specificity

      Expressed only in pancreas and colon.

    • Sequence similarities

      Belongs to the MIP/aquaporin (TC 1.A.8) family.

    • Domain

      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • AQP 8 antibody
        • AQP-8 antibody
        • AQP8 antibody
        • AQP8_HUMAN antibody
        • Aquaporin 8 antibody
        • Aquaporin-8 antibody
        • Aquaporin8 antibody
        see all

      Images

      • Western blot - Anti-AQP8 antibody [EPR8397] (ab133667)
        Western blot - Anti-AQP8 antibody [EPR8397] (ab133667)
        All lanes : Anti-AQP8 antibody [EPR8397] (ab133667) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : BxPC-3 cell lysate
        Lane 3 : Caco-2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 27 kDa

      • Flow Cytometry - Anti-AQP8 antibody [EPR8397] (ab133667)
        Flow Cytometry - Anti-AQP8 antibody [EPR8397] (ab133667)

        Flow cytometry analysis of HeLa cells labelling AQP8 with ab133667 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

      • OI-RD Scanning - Anti-AQP8 antibody [EPR8397] (ab133667)
        OI-RD Scanning - Anti-AQP8 antibody [EPR8397] (ab133667)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-AQP8 antibody [EPR8397] (ab133667)
        Anti-AQP8 antibody [EPR8397] (ab133667)

      References (1)

      Publishing research using ab133667? Please let us know so that we can cite the reference in this datasheet.

      ab133667 has been referenced in 1 publication.

      • Pellavio G  et al. HPV Infection Affects Human Sperm Functionality by Inhibition of Aquaporin-8. Cells 9:N/A (2020). PubMed: 32429588

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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