Recombinant
RabMAb

Recombinant Anti-AQP8 antibody [EPR8397] (ab133667)

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Overview

  • Product name

    Anti-AQP8 antibody [EPR8397]
    See all AQP8 primary antibodies

  • Description

    Rabbit monoclonal [EPR8397] to AQP8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    corresponding to Human AQP8 aa 100-200 (extracellular).
    Database link: O94778

  • Positive control

    • HeLa, BxPC-3 and Caco-2 cell lysates; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Aquaporin 8

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 4.60 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8397

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.

    • Tissue specificity

      Expressed only in pancreas and colon.

    • Sequence similarities

      Belongs to the MIP/aquaporin (TC 1.A.8) family.

    • Domain

      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • AQP 8 antibody
        • AQP-8 antibody
        • AQP8 antibody
        • AQP8_HUMAN antibody
        • Aquaporin 8 antibody
        • Aquaporin-8 antibody
        • Aquaporin8 antibody
        see all

      Images

      • Western blot - Anti-AQP8 antibody [EPR8397] (ab133667)
        Western blot - Anti-AQP8 antibody [EPR8397] (ab133667)
        All lanes : Anti-AQP8 antibody [EPR8397] (ab133667) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : BxPC-3 cell lysate
        Lane 3 : Caco-2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 27 kDa

      • Flow Cytometry - Anti-AQP8 antibody [EPR8397] (ab133667)
        Flow Cytometry - Anti-AQP8 antibody [EPR8397] (ab133667)

        Flow cytometry analysis of HeLa cells labelling AQP8 with ab133667 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.

      • Other - Anti-AQP8 antibody [EPR8397] (ab133667)
        Other - Anti-AQP8 antibody [EPR8397] (ab133667)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab133667 has not yet been referenced specifically in any publications.

      Publishing research using ab133667? Please let us know so that we can cite the reference in this datasheet.

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