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  1. Link

    aqua-block-buffer-ab166952.pdf

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Kits/ Lysates/ Other Kits IHC tools Block Protein Block
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Aqua Block Buffer (ab166952)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Overview

  • Product name

    Aqua Block Buffer
  • Tested applications

    Suitable for: Blocking, WBmore details
  • General notes

    Aqua Block Buffer is a non-mammalian blocking reagent supplied in PBS for immunoassays involving membrane or other solid phase material. This new reagent is optimized for superior performance in super sensitive assay systems, while utilizing proteins that do not interact with mammalian antibodies.

    Numerous solutions containing proteins are used and recommended for blocking of excess binding sites in ELISA, Western Blotting, Immunohistochemistry and other immunochemical applications. Aqua Block Buffer utilizes proteins that do not interact substantially with mammalian antibodies and therefore will display little or no background.

    How to Use:
    1. Add the blocking solution to the ELISA wells, blotting membrane or immuhistochemical slide.
    2. Incubate 30 minutes to 2 hours at either room temperature or 37°C.
    3. Continue with other reagents after the blocking according to your own protocol.

    NOTE: Using this blocker as a diluent with HRP conjugates may decrease your signal since this blocker contains sodium azide.

    Purity: Whole antiserum

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, Whole serum
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Block
    • Protein Block

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If a decrease in signal is noted, further dilutions of the solution may restore the signal.
Notes
Blocking
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If a decrease in signal is noted, further dilutions of the solution may restore the signal.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab166952? Please let us know so that we can cite the reference in this datasheet.

ab166952 has been referenced in 1 publication.

  • Vora SM  et al. Centrosomes are required for proper ß-catenin processing and Wnt response. Mol Biol Cell 31:1951-1961 (2020). PubMed: 32583737

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question




I have a small question regarding the choice of Sea Block Buffer (ab166951) or Aqua Block Buffer (ab166952) for use in ELISA. What is the difference between them and what should be the decisive factor of choice?









Dr. Byron Baron

Academic Researcher and Lecturer

Centre for Molecular Medicine and Biobanking

Faculty of Medicine and Surgery

University of Malta

Msida. MSD2080

Malta

Read More

Abcam community

Verified customer

Asked on Oct 28 2016

Answer





The Sea Block Buffer (ab166951) has been optimised for ELISA whereas the Aqua block buffer (ab166952) is specifically formulated for Western blot.


Read More

Abcam Scientific Support

Answered on Oct 28 2016

Question

Is the seablock plasma included in the aquablock buffer?

Read More

Abcam community

Verified customer

Asked on May 02 2014

Answer

The SeaBlock Plasma ab166953 is included in the AquaBlock buffer ab166952.

Read More

Kevin Hanson

Abcam Scientific Support

Answered on May 02 2014

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