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    aqua-block-buffer-ab166952.pdf

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Kits/ Lysates/ Other Kits IHC tools Block Protein Block
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Aqua Block Buffer (ab166952)

  • Datasheet
  • SDS
Submit a review Q&A (2)

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Overview

  • Product name

    Aqua Block Buffer
  • Tested applications

    Suitable for: Blocking, WBmore details
  • General notes

    Aqua Block Buffer is a non-mammalian blocking reagent supplied in PBS for immunoassays involving membrane or other solid phase material. This new reagent is optimized for superior performance in super sensitive assay systems, while utilizing proteins that do not interact with mammalian antibodies.

    Numerous solutions containing proteins are used and recommended for blocking of excess binding sites in ELISA, Western Blotting, Immunohistochemistry and other immunochemical applications. Aqua Block Buffer utilizes proteins that do not interact substantially with mammalian antibodies and therefore will display little or no background.

    How to Use:
    1. Add the blocking solution to the ELISA wells, blotting membrane or immuhistochemical slide.
    2. Incubate 30 minutes to 2 hours at either room temperature or 37°C.
    3. Continue with other reagents after the blocking according to your own protocol.

    NOTE: Using this blocker as a diluent with HRP conjugates may decrease your signal since this blocker contains sodium azide.

    Purity: Whole antiserum

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Block
    • Protein Block

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab166952 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Blocking
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If a decrease in signal is noted, further dilutions of the solution may restore the signal.
    Notes
    Blocking
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If a decrease in signal is noted, further dilutions of the solution may restore the signal.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab166952? Please let us know so that we can cite the reference in this datasheet.

    ab166952 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question




    I have a small question regarding the choice of Sea Block Buffer (ab166951) or Aqua Block Buffer (ab166952) for use in ELISA. What is the difference between them and what should be the decisive factor of choice?









    Dr. Byron Baron

    Academic Researcher and Lecturer

    Centre for Molecular Medicine and Biobanking

    Faculty of Medicine and Surgery

    University of Malta

    Msida. MSD2080

    Malta

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2016

    Answer





    The Sea Block Buffer (ab166951) has been optimised for ELISA whereas the Aqua block buffer (ab166952) is specifically formulated for Western blot.


    Read More

    Abcam Scientific Support

    Answered on Oct 28 2016

    Question

    Is the seablock plasma included in the aquablock buffer?

    Read More

    Abcam community

    Verified customer

    Asked on May 02 2014

    Answer

    The SeaBlock Plasma ab166953 is included in the AquaBlock buffer ab166952.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on May 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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