Aqua Block Buffer (ab166952)
Overview
-
Product name
Aqua Block Buffer -
Tested applications
Suitable for: Blocking, WBmore details -
General notes
Aqua Block Buffer is a non-mammalian blocking reagent supplied in PBS for immunoassays involving membrane or other solid phase material. This new reagent is optimized for superior performance in super sensitive assay systems, while utilizing proteins that do not interact with mammalian antibodies.
Numerous solutions containing proteins are used and recommended for blocking of excess binding sites in ELISA, Western Blotting, Immunohistochemistry and other immunochemical applications. Aqua Block Buffer utilizes proteins that do not interact substantially with mammalian antibodies and therefore will display little or no background.
How to Use:
1. Add the blocking solution to the ELISA wells, blotting membrane or immuhistochemical slide.
2. Incubate 30 minutes to 2 hours at either room temperature or 37°C.
3. Continue with other reagents after the blocking according to your own protocol.NOTE: Using this blocker as a diluent with HRP conjugates may decrease your signal since this blocker contains sodium azide.
Purity: Whole antiserum
Properties
-
Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, Whole serum -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Blocking |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If
a decrease in signal is noted, further dilutions of the solution may restore the
signal.
|
| Notes |
|---|
|
Blocking
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If a decrease in signal is noted, further dilutions of the solution may restore the signal. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab166952 has been referenced in 1 publication.
- Vora SM et al. Centrosomes are required for proper ß-catenin processing and Wnt response. Mol Biol Cell 31:1951-1961 (2020). PubMed: 32583737