Anti-Aquaporin 1 antibody [1/22] (ab9566)

Reviews (6)Q&A (8)References (34)

Overview

  • Product name
    Anti-Aquaporin 1 antibody [1/22]
    See all Aquaporin 1 primary antibodies
  • Description
    Mouse monoclonal [1/22] to Aquaporin 1
  • Host species
    Mouse
  • Specificity
    This antibody recognises Aquaporin 1
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Fr, ICC, Flow Cyt, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow
  • Immunogen

    Synthetic peptide:

    GQVEEYDLDDDINSRVEMKPK

    , corresponding to amino acids 249-269 of Aquaporin 1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope
    Intracellular C-terminal AQP 1 epitope (active channel loop)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    1/22
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.
IHC-P 1/100. PubMed: 17446713Antigen retrieval, such as heating of slides in 10 mM citrate buffer, pH 6.00 or proteolytic pre-treatment of tissues may increase the staining intensity. The recommended time of incubation is 60 minutes at room temperature.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18418349

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Immunohistochemical staining of aquaporin 1 in rat brain.
  • Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)

    Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566. 

  • Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)
    Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa
    Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

References

This product has been referenced in:
  • Wagoner MD  et al. Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biol Open 7:N/A (2018). Read more (PubMed: 29685994) »
  • González-Marrero I  et al. Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na?, K? ATPase in Hypertensive Rats. Cells 7:N/A (2018). Read more (PubMed: 30428541) »
See all 34 Publications for this product

Publishing research using ab9566? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 14 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody [1/22]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab94674 (100X Citrate Buffer pH 6.0)
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
10%NBF
Read More

Mr. David Ivancic

Verified customer

Submitted May 04 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody [1/22]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Intervertebral Disc)
Antigen retrieval step
None
Permeabilization
No
Specification
Intervertebral Disc
Blocking step
1% BSA in 75% TBS 25% Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
Fixative
10% Neutral Buffered Formalin
Read More

Abcam user community

Verified customer

Submitted Jul 14 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody [1/22]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Human Tissue sections (Corneal endothelium)
Specification
Corneal endothelium
Permeabilization
Yes - Triton 0,3% - 5 minutes
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 14 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Aquaporin 1 antibody [1/22]

 Poor
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Human Cell (kidney, proximal tubule)
Specification
kidney, proximal tubule
Permeabilization
Yes - 1 % Triton X
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 09 2014

Western blot abreview for Anti-Aquaporin 1 antibody [1/22]

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (kidney, proximal tubule)
Specification
kidney, proximal tubule
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Nov 20 2013

Question

Bonjour Monsieur,
Merci de votre mail. Je voudrais acheter l'anti-aquaporin 1 ab9566 pour l'immunohistochimie. Comme sur le tissue, on ne peut pas savoir si le marquage est bien spécifique. Avant de le tester, on cherche toujours pour assurer la spécificité de l'anticorps, et sur le western blot de votre fiche je vois plusieurs bandes, c'est la raison pour la quelle, je voudrais avoir votre expérience.
je vous remercie pour votre aide
Bien cordialement

Read More
Answer

Merci de nous avoir contactés.

La source de l'anticorps s'excuse du délai de la réponse du à des problèmes de connexion internet et la perte de messages.
Ils viennent de m'informer que les bandes dites non-spécifiques avec le ab9566 correspondent probablement à des produits de dégradations de cette protéine de grande taille.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Question

questions about ab9566

Read More
Answer

Merci de nous avoir contactés.

J'ai essayé de vous joindre par téléphone mais malheureusement sans succès.
J'ai compris que vous aviez des questions sur l'anti-aquaporin 1 ab9566.

N'hésitez pas à me faire part de vos questions en répondant à cet email, je serai ravi de vous venir en aide.

Read More

Question

Dear technical support,

Recently I purchased your anti-aquaporin-1 antibody, ab9566. I would also like to purchase your anti-aquaporin 2 antibody ab15116, but first I would like to know if you think they'd cross-react if used on the same immunohistochemistry tissue slide? Or would there be discrete staining?

The AQP1 antibody was raised in mouse, the AQP2 antibody from rabbit. I'm looking to use them both on rat and mouse tissue, which they say they react with successfully.

Any technical help you could provide would be much appreciated.

Thanks,

Luke








Mr Luke Shelton (PhD Research Student)





2.64 The Sherrington Building
MRC Centre for Drug Safety Science
Molecular & Clinical Pharmacology
Ashton Street,
Liverpool,

United Kingdom,

L69 3GE

Read More
Answer

Thank you for your enquiry.

I am pleased to help answer your questions and apologize for the delay in responding. I have been waiting for confirmation of some information from our sources.

The Immunogen for ab9566Anti-Aquaporin 1 antibody [1/22] is provided:

GQVEEYDLDDDINSRVEMKPK

This has 9% alignment to mouse Aquaporin 2. I am sorry to confirm that the antibody has not specifically been tested for crossreactivity to aquaporin 2 in the laboratories. However, the alignment to aquaporin 1is very low and we would not expect this antibody to cross react with aquaporin 2

For ab15116 Anti-Aquaporin 2 antibody,the immunogen for this antibody is a peptide comprised of a 15 amino acid sequence from near the C-terminus of aquaporin 2. The 15 amino acid are within the last 50 amino acids of the sequence (NP_037041). Again, I am sorry this antibody has not been specificallytestedfor crossreactivity to aquaporin 1. However, there is again very low alignment to the immunogen to aquaporin 1 so we would not expect it todetect aquaporin 1.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody [1/22]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase
Permeabilization
No
Blocking step
Peroxidase Blocking Solution (Dako) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 06 2009

Question

There appears to be one amino acid difference between the immunogen sequence on the datasheet and the BLAST sequence. Is this the correct immunogen, or is this a typo?

Read More
Answer

Thank you for your enquiry. I contacted the authors who made the antibody and Prof. Peter Nemeth told me, "We used the DDD sequence by technical difficulties of peptide synthesis. The one amino acid difference in antigen didn’t influence the specificity of our antibody according to the results of different testing techniques." I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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1-10 of 14 Abreviews or Q&A

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