Anti-Aquaporin 1 antibody [1/22] (ab9566)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/ab9566-17269-anti-aquaporin-1-antibody-1-22-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)")
Key features and details
- Mouse monoclonal [1/22] to Aquaporin 1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Aquaporin 1 antibody [1/22]
See all Aquaporin 1 primary antibodies -
Description
Mouse monoclonal [1/22] to Aquaporin 1 -
Host species
Mouse -
Specificity
This antibody recognises Aquaporin 1 -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P RatWB Human
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Immunogen
Synthetic peptide:
GQVEEYDLDDDINSRVEMKPK
, corresponding to amino acids 249-269 of Aquaporin 1. -
Epitope
Intracellular C-terminal AQP 1 epitope (active channel loop) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1/22 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| IHC-P |
Rat
|
| WB |
Human
|
| All applications |
Sheep
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| IHC-P | (9) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1-2µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
Tissue specificity
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 358 Human
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: P29975 Rat
- Unigene: 76152 Human
- Unigene: 1618 Rat
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Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical staining of aquaporin 1 in rat brain.
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![Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/ab9566-17267-anti-aquaporin-1-antibody-1-22-flow-cytometry.jpg)
Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/ab9566-279831-anti-aquaporin-1-antibody-1-22-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566.
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![Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/ab9566-17268-anti-aquaporin-1-antibody-1-22-western-blot.jpg)
Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab9566 has been referenced in 40 publications.
- Gee MT et al. Expression of SLC4A11 protein in mouse and rat medulla: a candidate transporter involved in outer medullary ammonia recycling. Physiol Rep 7:e14089 (2019). PubMed: 31124301
- Luo L et al. Decreased miR-320 expression is associated with breast cancer progression, cell migration, and invasiveness via targeting Aquaporin 1. Acta Biochim Biophys Sin (Shanghai) 50:473-480 (2018). PubMed: 29538612
- Wagoner MD et al. Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biol Open 7:N/A (2018). PubMed: 29685994
- González-Marrero I et al. Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na?, K? ATPase in Hypertensive Rats. Cells 7:N/A (2018). PubMed: 30428541
- Palacio-Mancheno PE et al. Hyperosmolarity induces notochordal cell differentiation with aquaporin3 upregulation and reduced N-cadherin expression. J Orthop Res 36:788-798 (2018). PubMed: 28853179