Anti-Aquaporin 1 antibody [1/22] (ab9566)
- Datasheet
- References (33)
- Protocols
Overview
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Product nameAnti-Aquaporin 1 antibody [1/22]
See all Aquaporin 1 primary antibodies -
DescriptionMouse monoclonal [1/22] to Aquaporin 1
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Host speciesMouse
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SpecificityThis antibody recognises Aquaporin 1
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Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, ICC, Flow Cyt, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow
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Immunogen
Synthetic peptide:
GQVEEYDLDDDINSRVEMKPK
, corresponding to amino acids 249-269 of Aquaporin 1. -
EpitopeIntracellular C-terminal AQP 1 epitope (active channel loop)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number1/22
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IsotypeIgG2b
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/100. PubMed: 17446713Antigen retrieval, such as heating of slides in 10 mM citrate buffer, pH 6.00 or proteolytic pre-treatment of tissues may increase the staining intensity. The recommended time of incubation is 60 minutes at room temperature. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1-2µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| IHC-FoFr | Use at an assay dependent concentration. PubMed: 18418349 |
Target
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FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
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Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
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DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/ab9566_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical staining of aquaporin 1 in rat brain. -
![Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/Aquaporin-1-Primary-antibodies-ab9566-5.jpg)
Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/ab9566-279831-ab9566IHCJP.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566.
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![Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.com/ps/products/9/ab9566/Images/Aquaporin-1-Primary-antibodies-ab9566-4.jpg)
Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
References
This product has been referenced in:
- Wagoner MD et al. Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells. Biol Open 7:N/A (2018). Read more (PubMed: 29685994) »
- Luo LM et al. The association between aquaporin-1 expression, microvessel density and the clinicopathological features of hepatocellular carcinoma. Oncol Lett 14:7077-7084 (2017). Read more (PubMed: 29344137) »
