Anti-Aquaporin 1 antibody [7D11] (ab11025)

Reviews (1)Q&A (1)References (4)

Overview

  • Product name
    Anti-Aquaporin 1 antibody [7D11]
    See all Aquaporin 1 primary antibodies
  • Description
    Mouse monoclonal [7D11] to Aquaporin 1
  • Host species
    Mouse
  • Specificity
    ab11025 recognises an epitope within an extracellular region of the water-specific channel aquaporin 1.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 198-208 of Rat Aquaporin 1.

  • Positive control
    • Kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    7D11
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/500 - 1/1000.
WB 1/1000 - 1/2000. Predicted molecular weight: 32.28 kDa.
ELISA 1/1000 - 1/10000.

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

References

This product has been referenced in:
  • George SK  et al. Potential Use of Autologous Renal Cells from Diseased Kidneys for the Treatment of Renal Failure. PLoS One 11:e0164997 (2016). Read more (PubMed: 27776163) »
  • Kang HM  et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). Read more (PubMed: 26776520) »
See all 4 Publications for this product

Publishing research using ab11025? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody [7D11]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
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Vlady Pavlova

Verified customer

Submitted Aug 16 2006

Question

BATCH NUMBER 97965 ORDER NUMBER 6996 DESCRIPTION OF THE PROBLEM No staining SAMPLE rat kidney frozen section PRIMARY ANTIBODY abcam11025/rat/1:50/in blocking solution/2h/3xPBS 5min DETECTION METHOD Fluorescence microscopy POSITIVE AND NEGATIVE CONTROLS USED positive control for secondary Ab Na/K ATPase negative secondary only, all at the same frozen sections ANTIBODY STORAGE CONDITIONS 4 degree FIXATION OF SAMPLE Ethanol/Methanol/Acetone ANTIGEN RETRIEVAL nil PERMEABILIZATION STEP nil BLOCKING CONDITIONS 1%BSA+10%FCS 30 min SECONDARY ANTIBODY [ a competitor] donkey anti-mouse FITC IgG/PBS/1:100/1h/3xPBS 5min HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 10 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? fixation,dilutions/incubation time ADDITIONAL NOTES The full PO number is 4512006996

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Answer

I'm sorry to hear you are experiencing problems with ab11025. This antibody has not been tested in immunofluorescence and this may be the reason for the problem. I would recommend trying a biotin-ABC-DAB detection system which is more sensitive that IF (indeed I would recommend more powerful fluorochromes in general, such as Alexa or Cy3 antibodies for immunofluorescence detection). I would also recommend the following modifications: -incubate the antibody for longer, such as overnight at 4C -use tritonx100 in the dilution buffer for the blocking agent and the primary and secondary antibodies -you did not mention the fixation time or temperature, can I please confirm that the alcohol should be ice cold (pre-cooled at -20C then incubation should be at RT) and the fixation time should be around 10min. Please do not hesitate to contact us again if you still experience problems,

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