Anti-Aquaporin 1 antibody [7D11] (ab11025)
- Datasheet
- References (4)
- Protocols
Overview
-
Product nameAnti-Aquaporin 1 antibody [7D11]
See all Aquaporin 1 primary antibodies -
DescriptionMouse monoclonal [7D11] to Aquaporin 1
-
Host speciesMouse
-
Specificityab11025 recognises an epitope within an extracellular region of the water-specific channel aquaporin 1.
-
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ELISAmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide, corresponding to amino acids 198-208 of Rat Aquaporin 1.
-
Positive control
- Kidney tissue.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityProtein G purified
-
Purification notesPurified from tissue culture supernatant.
-
ClonalityMonoclonal
-
Clone number7D11
-
MyelomaSp2/0-Ag14
-
IsotypeIgG2b
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab11025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | 1/500 - 1/1000. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 32.28 kDa. | |
| ELISA | 1/1000 - 1/10000. |
Target
-
FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
-
Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
-
Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
-
DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
-
Cellular localizationMembrane.
- Information by UniProt
-
Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Datasheets and documents
References
This product has been referenced in:
- George SK et al. Potential Use of Autologous Renal Cells from Diseased Kidneys for the Treatment of Renal Failure. PLoS One 11:e0164997 (2016). Read more (PubMed: 27776163) »
- Kang HM et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). Read more (PubMed: 26776520) »
