Overview

  • Product name

  • Description

    Rabbit polyclonal to Aquaporin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Chicken, Cow, Dog, Chinese hamster
  • Immunogen

    Synthetic peptide:

    N-MASEFKKKLF

    , corresponding to N terminal amino acids 1-10 of Rat Aquaporin 1 (NP_036910.1).

  • Positive control

    • Rat kidney tissue and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/4000. Predicted molecular weight: 29 kDa. 0.5 µg/ml of ab125041 was sufficient for detection of Aquaporin 1 in 10µg of Rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
ICC/IF 1/200 - 1/400.

Target

  • Function

    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity

    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Images

  • ab125041, at 1/200, staining Aquaporin 1 in Rat kidney tissue by Immunofluorescence.

References

ab125041 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Sample
Chicken Tissue lysate - whole (embryonic head)
Total protein in input
200 µg
Immuno-precipitation step
Other - Protein A dynabeads
Specification
embryonic head

Abcam user community

Verified customer

Submitted Jan 08 2019

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