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A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.
Our Abpromise guarantee covers the use of ab15080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 1 peptide (ab25381).|
|ELISA||1/10000 - 1/50000.|
|ICC||Use at an assay dependent concentration. PubMed: 22174795|
ab15080 staining Aquaporin 1 in the intervertebral disc (nucleus pulposus) of the cow cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
ab15080 at 1/500 dilution, staining Aquaporin 1 in sheep duodenum section by immunohistochemistry (Frozen sections). Sections were paraformaldehyde fixed and incubated with ab15080, for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit Ig, diluted 1/400 was used as the secondary antibody.
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