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    aquaporin-1-antibody-ab15080.pdf

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Signal Transduction Metabolism Plasma Membrane Channels
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Anti-Aquaporin 1 antibody (ab15080)

  • Datasheet
  • SDS
Reviews (16)Q&A (7)References (42)

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ELISA - Anti-Aquaporin 1 antibody (ab15080)

    Key features and details

    • Rabbit polyclonal to Aquaporin 1
    • Suitable for: ELISA
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Aquaporin 1 protein (ab114210)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Aquaporin 1 antibody
      See all Aquaporin 1 primary antibodies
    • Description

      Rabbit polyclonal to Aquaporin 1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ELISAmore details
    • Immunogen

      A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Aquaporin 1 protein (ab114210)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab15080 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.

    Target

    • Function

      Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
    • Tissue specificity

      Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
    • Sequence similarities

      Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • Domain

      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P29972 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • AQP 1 antibody
      • AQP CHIP antibody
      • AQP-1 antibody
      • AQP1 antibody
      • AQP1_HUMAN antibody
      • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
      • aquaporin 1 (Colton blood group) antibody
      • Aquaporin CHIP antibody
      • Aquaporin-1 antibody
      • Aquaporin-CHIP antibody
      • Aquaporin1 antibody
      • Channel forming integral protein 28kDa antibody
      • Channel like integral membrane protein 28 kDa antibody
      • CHIP 28 antibody
      • CHIP28 antibody
      • CO antibody
      • Colton blood group antibody
      • Growth factor induced delayed early response protein antibody
      • MGC26324 antibody
      • Urine water channel antibody
      • Water channel protein CHIP 29 antibody
      • Water channel protein CHIP29 antibody
      • Water channel protein for red blood cells and kidney proximal tubule antibody
      see all

    Images

    • ELISA - Anti-Aquaporin 1 antibody (ab15080)
      ELISA - Anti-Aquaporin 1 antibody (ab15080)

      Antibody titer determination against Aquaporin-1 using pre-immunised serum, Antiserum and Affinity purified Anti-Aquaporin 1 (Cat: ab15080).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (42)

    Publishing research using ab15080? Please let us know so that we can cite the reference in this datasheet.

    ab15080 has been referenced in 42 publications.

    • Wang J  et al. Bax inhibitor 1 preserves mitochondrial homeostasis in acute kidney injury through promoting mitochondrial retention of PHB2. Theranostics 10:384-397 (2020). PubMed: 31903127
    • McCrimmon A  et al. Comprehensive assessment of mitochondrial respiratory function in freshly isolated nephron segments. Am J Physiol Renal Physiol 318:F1237-F1245 (2020). PubMed: 32223308
    • Lv Y  et al. 1a,25-dihydroxyvitamin D3 attenuates oxidative stress-induced damage in human trabecular meshwork cells by inhibiting TGFß-SMAD3-VDR pathway. Biochem Biophys Res Commun 516:75-81 (2019). PubMed: 31196627
    • Dalghi MG  et al. Expression and distribution of PIEZO1 in the mouse urinary tract. Am J Physiol Renal Physiol 317:F303-F321 (2019). PubMed: 31166705
    • Chamanza R  et al. Normal Anatomy, Histology, and Spontaneous Pathology of the Kidney, and Selected Renal Biomarker Reference Ranges in the Cynomolgus Monkey. Toxicol Pathol 47:612-633 (2019). PubMed: 31409264
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 23 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0 (ab64236 )
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 23 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Aquaporin 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Kidney primary culture)
    Permeabilization
    No
    Specification
    Kidney primary culture
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 07 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    sea blcok as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Mr. David Ivancic

    Verified customer

    Submitted Jan 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Colon
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Mr. David Ivancic

    Verified customer

    Submitted Jul 02 2019

    IHC - Wholemount abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Tissue (Lens Capsule)
    Specification
    Lens Capsule
    Read More

    Mr. Manuel Chacon

    Verified customer

    Submitted Aug 25 2015

    1-10 of 23 Abreviews or Q&A

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