Anti-Aquaporin 1 antibody (ab15080)
- Datasheet
- References (22)
- Protocols
Overview
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Product nameAnti-Aquaporin 1 antibody
See all Aquaporin 1 primary antibodies -
DescriptionRabbit polyclonal to Aquaporin 1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Fr, ICCmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Cow, Dog, Human, Pig
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Immunogen
A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.
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Positive control
- rat kidney lysate, rat heart lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: PBS -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/500. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 1 peptide (ab25381). | |
| ELISA | 1/10000 - 1/50000. | |
| IHC-Fr | 1/500. | |
| ICC | Use at an assay dependent concentration. PubMed: 22174795 |
Target
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FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
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Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
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Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
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DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody (ab15080)This image is courtesy of an anonymous Abreviewab15080 staining Aquaporin 1 in human kidney tubule tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde. Antigen retrieval was by heat mediation in citrate buffer (20 minutes at 100°C). Samples were incubated with primary antibody 1/2000(background reducer diluent) for 20 minutes at 25°C. An undiluted HRP polymer Goat anti mouse/rabbit monoclonal IgG was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody (ab15080)This image is courtesy of an Abreview by Robab15080 staining Aquaporin 1 in the intervertebral disc (nucleus pulposus) of the cow cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
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Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody (ab15080)This image is courtesy of an Abreview submitted by Prof Marcin Arciszewskiab15080 staining Aquaporin1 in porcine jejunum by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody diluted 1/500 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal IgG whole molecule to rabbit, diluted 1/400 was used as the secondary. -
Immunocytochemistry - Anti-Aquaporin 1 antibody (ab15080)Image from Abreu-Rodríguez I et al., PLoS One. 2011;6(12):e28385. Epub 2011 Dec 7. Fig 1.; doi:10.1371/journal.pone.0028385; December 7, 2011, PLoS ONE 6(12): e28385.ab15080 staining Aquaporin 1 in rat 9L (gliosarcoma) cells by Immunocytochemistry.
Cells were kept under normoxic conditions (upper panel) or exposed to hypoxia (lower panel). Cells were then fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in PBS, before being blocked with 10% fetal calf serum + 1 mg/ml BSA in PBS overnight at 4°C. Samples were subsequently incubated with primary antibody (1/500 in diluent) and a peroxidase-conjugated goat anti-rabbit IgG was used as a secondary antibody. Samples were stained using DAB and counter-stained with hematoxylin. -
Immunohistochemistry (Frozen sections) - Anti-Aquaporin 1 antibody (ab15080)This image is courtesy of an Abreview submitted by Prof Marcin Arciszewskiab15080 at 1/500 dilution, staining Aquaporin 1 in sheep duodenum section by immunohistochemistry (Frozen sections). Sections were paraformaldehyde fixed and incubated with ab15080, for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit Ig, diluted 1/400 was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Christov M et al. Inducible podocyte-specific deletion of CTCF drives progressive kidney disease and bone abnormalities. JCI Insight 3:N/A (2018). Read more (PubMed: 29467330) »
- Wang Y et al. Cytoprotective effect of aquaporin 1 against lipopolysaccharide-induced apoptosis and inflammation of renal epithelial HK-2 cells. Exp Ther Med 15:4243-4252 (2018). Read more (PubMed: 29731819) »
