Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Cow, Dog, Human, Pig
  • Immunogen

    A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.

  • Positive control
    • rat kidney lysate, rat heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab15080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 1 peptide (ab25381).
ELISA 1/10000 - 1/50000.
IHC-Fr 1/500.
ICC Use at an assay dependent concentration. PubMed: 22174795

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Images

  • ab15080 staining Aquaporin 1 in human kidney tubule tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde. Antigen retrieval was by heat mediation in citrate buffer (20 minutes at 100°C). Samples were incubated with primary antibody 1/2000(background reducer diluent) for 20 minutes at 25°C. An undiluted HRP polymer Goat anti mouse/rabbit monoclonal IgG was used as secondary antibody.

    See Abreview

  • ab15080 staining Aquaporin 1 in the intervertebral disc (nucleus pulposus) of the cow cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).

    See Abreview

  • ab15080 staining Aquaporin1 in porcine jejunum by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody diluted 1/500 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal IgG whole molecule to rabbit, diluted 1/400 was used as the secondary.

    See Abreview

  • ab15080 staining Aquaporin 1 in rat 9L (gliosarcoma) cells by Immunocytochemistry.

    Cells were kept under normoxic conditions (upper panel) or exposed to hypoxia (lower panel). Cells were then fixed with paraformaldehyde, permeabilized in 0.1% Triton X-100 in PBS, before being blocked with 10% fetal calf serum + 1 mg/ml BSA in PBS overnight at 4°C. Samples were subsequently incubated with primary antibody (1/500 in diluent) and a peroxidase-conjugated goat anti-rabbit IgG was used as a secondary antibody. Samples were stained using DAB and counter-stained with hematoxylin.
  • ab15080 at 1/500 dilution, staining Aquaporin 1 in sheep duodenum section by immunohistochemistry (Frozen sections). Sections were paraformaldehyde fixed and incubated with ab15080, for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit Ig, diluted 1/400 was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Christov M  et al. Inducible podocyte-specific deletion of CTCF drives progressive kidney disease and bone abnormalities. JCI Insight 3:N/A (2018). Read more (PubMed: 29467330) »
  • Wang Y  et al. Cytoprotective effect of aquaporin 1 against lipopolysaccharide-induced apoptosis and inflammation of renal epithelial HK-2 cells. Exp Ther Med 15:4243-4252 (2018). Read more (PubMed: 29731819) »
See all 22 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-7 of 7 Abreviews

Application
IHC - Wholemount
Sample
Human Tissue (Lens Capsule)
Specification
Lens Capsule

Mr. Manuel Chacon

Verified customer

Submitted Aug 25 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Sample
Cow Cell (Intervertebral disc (nucleus pulposus))
Specification
Intervertebral disc (nucleus pulposus)
Permeabilization
Yes - 0.5% Triton-X 100
Fixative
Paraformaldehyde

Rob Valued Customer

Verified customer

Submitted Jul 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kideny, tubules)
Specification
kideny, tubules
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, 100C, 20 min
Permeabilization
No

Abcam user community

Verified customer

Submitted Nov 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (ENS from the porcine jejunum)
Specification
ENS from the porcine jejunum
Fixative
Paraformaldehyde
Permeabilization
No

Prof. Marcin Arciszewski

Verified customer

Submitted Nov 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (ENS of the jejunum)
Specification
ENS of the jejunum
Fixative
Paraformaldehyde
Permeabilization
No

Prof. Marcin Arciszewski

Verified customer

Submitted Oct 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (duodenum)
Specification
duodenum
Fixative
Paraformaldehyde
Permeabilization
No

Prof. Marcin Arciszewski

Verified customer

Submitted Oct 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up