Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from near the C-terminus of human Aquaporin 1, identical to the related mouse sequence.

  • Positive control
    • rat kidney.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 1-3µg for 106 cells.

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Images

  • All lanes : Anti-Aquaporin 1 antibody (ab65837) at 0.5 µg/ml

    Lane 1 : Rat Kidney Tissue Lysate with 5% Non-fat Milk/ TBS
    Lane 2 : Rat LungTissue Lysate with 5% Non-fat Milk/ TBS
    Lane 3 : SMMC Cell Lysate with 5% Non-fat Milk/ TBS

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 29 kDa

  • Aquaporin 1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Overlay histogram showing U20S cells stained with ab65837 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 1 Antibody (ab65837,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. 

  • Aquaporin 1 was detected in frozen section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Aquaporin 1 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65837 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Anti-Aquaporin 1 antibody (ab65837) at 2 µg/ml + rat kidney lysate

    Predicted band size: 29 kDa
    Observed band size: 39 kDa (why is the actual band size different from the predicted?)



    Aquaporin 1 is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites - it is likely that the difference in size observed is related to protein glycosylation.

References

This product has been referenced in:
  • Tao YS  et al. Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats. BMC Nephrol 19:63 (2018). Read more (PubMed: 29540150) »
  • Souma T  et al. Luminal alkalinization attenuates proteinuria-induced oxidative damage in proximal tubular cells. J Am Soc Nephrol 22:635-48 (2011). ICC/IF ; Human . Read more (PubMed: 21372211) »

See all 4 Publications for this product

Customer reviews and Q&As

Lamento muchísimo las molestias ocasionadas con este anticuerpo. También te agradezco que nos comuniquéis los problemas experimentados con nuestros productos y te animo a que sigáis haciéndolo en el futuro, aunque espero que no se repitan. Muchas graci...

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