Product nameAnti-Aquaporin 1 antibody
See all Aquaporin 1 primary antibodies
DescriptionRabbit polyclonal to Aquaporin 1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Sheep, Cow, Orangutan
Synthetic peptide corresponding to Human Aquaporin 1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Aquaporin 1 protein (ab114210) can be used as a positive control in WB. This antibody gave a positive signal in both HEK293 and Jurkat whole cell lysates as well as the following tissue lysates: Human pancreas; Human heart; Human skeletal muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab87845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).|
FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
- Information by UniProt
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
All lanes : Anti-Aquaporin 1 antibody (ab87845) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human pancreas tissue lysate - total protein (ab29816)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Human Aquaporin-1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab87845 has not yet been referenced specifically in any publications.