Recombinant
RabMAb

Recombinant Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

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Overview

  • Product name

    Anti-Aquaporin 1 antibody [EPR11588(B)]
    See all Aquaporin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11588(B)] to Aquaporin 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Aquaporin 1 aa 250-350. The exact sequence is proprietary.
    Database link: P29972

  • Positive control

    • Human fetal kidney, fetal lung and fetal heart lysates; Human kidney and lung tissues. ICC/IF: HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11588(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

    • Tissue specificity

      Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.

    • Sequence similarities

      Belongs to the MIP/aquaporin (TC 1.A.8) family.

    • Domain

      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AQP 1 antibody
      • AQP CHIP antibody
      • AQP-1 antibody
      • AQP1 antibody
      • AQP1_HUMAN antibody
      • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
      • aquaporin 1 (Colton blood group) antibody
      • Aquaporin CHIP antibody
      • Aquaporin-1 antibody
      • Aquaporin-CHIP antibody
      • Aquaporin1 antibody
      • Channel forming integral protein 28kDa antibody
      • Channel like integral membrane protein 28 kDa antibody
      • CHIP 28 antibody
      • CHIP28 antibody
      • CO antibody
      • Colton blood group antibody
      • Growth factor induced delayed early response protein antibody
      • MGC26324 antibody
      • Urine water channel antibody
      • Water channel protein CHIP 29 antibody
      • Water channel protein CHIP29 antibody
      • Water channel protein for red blood cells and kidney proximal tubule antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      All lanes : Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal lung lysate
      Lane 3 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

      Formalin-fixed, paraffin-embedded human colon tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

      Formalin-fixed, paraffin-embedded human breast tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

      Formalin-fixed, paraffin-embedded human kidney tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

      Formalin-fixed, paraffin-embedded human lung tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
      Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)This image is courtesy of an Abreview submitted by Kirk McManus.

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Aquaporin 1 with ab168387 at a dilution of 1/200. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

      The antibody produces a strong, membrane-associated labelling pattern in both MeOH and PFA fixed samples.

      See Abreview

    References

    This product has been referenced in:

    • Palethorpe HM  et al. The Aquaporin 1 Inhibitor Bacopaside II Reduces Endothelial Cell Migration and Tubulogenesis and Induces Apoptosis. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29495367) »
    • Luo H  et al. Aquaporin 1 gene deletion affects the amniotic fluid volume and composition as well as the expression of other aquaporin water channels in placenta and fetal membranes. Clin Chim Acta 482:161-165 (2018). WB . Read more (PubMed: 29626438) »
    See all 7 Publications for this product

    Publishing research using ab168387? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Aquaporin 1 antibody [EPR11588(B)]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (cervical cancer cells)
    Permeabilization
    Yes - PBA
    Specification
    cervical cancer cells
    Preparation
    Cell harvesting/tissue preparation method: HeLa cells were detached with standard trypsinization, washed with PBS, fixed and stored at -20°C until use. a
    Sample buffer: PBA: 0,1% PBS tween 20 + 1% BSA
    Fixation
    4% PFA for 10 min and 70% Ethanol
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 07 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Aquaporin 1 antibody [EPR11588(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human embryonic kidney)
    Permeabilization
    Yes - 0.25% Triton X
    Specification
    Human embryonic kidney
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Dr. Nils Lindstrom

    Verified customer

    Submitted Oct 07 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Aquaporin 1 antibody [EPR11588(B)]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 29 2016

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