Name: Anti-Aquaporin 1 antibody [EPR11588(B)]
Brand: RabMAb
SKU: ab168387
Rating: 4 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11588(B)] to Aquaporin 1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-Aquaporin 1 antibody [EPR11588(B)]
See all Aquaporin 1 primary antibodies
Description

Rabbit monoclonal [EPR11588(B)] to Aquaporin 1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Aquaporin 1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P29972
Positive control
- Human fetal kidney, fetal lung and fetal heart lysates; Human kidney and lung tissues. ICC/IF: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11588(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-Aquaporin 1 antibody [EPR11588(B)] - BSA and Azide free (ab178352)
- Alexa Fluor® 647 Anti-Aquaporin 1 antibody [EPR11588(B)] (ab225225)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Aquaporin 1 protein (ab114210)
Related Products
- Recombinant Human Aquaporin 1 protein (ab114210)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
ICC	Human
IHC-P	Human
WB	Human
All applications	Mouse Rat

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for IP.

Function

Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Tissue specificity

Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Sequence similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family.
Domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Cellular localization

Membrane.
Target information above from: UniProt accession P29972 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
- Unigene: 18625 Mouse
- Unigene: 1618 Rat
see all
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
- AQP1 antibody
- AQP1_HUMAN antibody
- aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
- aquaporin 1 (Colton blood group) antibody
- Aquaporin CHIP antibody
- Aquaporin-1 antibody
- Aquaporin-CHIP antibody
- Aquaporin1 antibody
- Channel forming integral protein 28kDa antibody
- Channel like integral membrane protein 28 kDa antibody
- CHIP 28 antibody
- CHIP28 antibody
- CO antibody
- Colton blood group antibody
- Growth factor induced delayed early response protein antibody
- MGC26324 antibody
- Urine water channel antibody
- Water channel protein CHIP 29 antibody
- Water channel protein CHIP29 antibody
- Water channel protein for red blood cells and kidney proximal tubule antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

All lanes : Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal lung lysate
Lane 3 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Formalin-fixed, paraffin-embedded human colon tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Formalin-fixed, paraffin-embedded human breast tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Formalin-fixed, paraffin-embedded human lung tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Publishing research using ab168387? Please let us know so that we can cite the reference in this datasheet.

ab168387 has been referenced in 12 publications.

Wu J et al. Gene Therapy for Glaucoma by Ciliary Body Aquaporin 1 Disruption Using CRISPR-Cas9. Mol Ther 28:820-829 (2020). PubMed: 31981492
Zhang G et al. Based on Histogram Analysis: ADCaqp Derived from Ultra-high b-Value DWI could be a Non-invasive Specific Biomarker for Rectal Cancer Prognosis. Sci Rep 10:10158 (2020). PubMed: 32576929
Nam SA et al. Graft immaturity and safety concerns in transplanted human kidney organoids. Exp Mol Med 51:1-13 (2019). PubMed: 31776328
Serman Y et al. Emerging new role of NFAT5 in inducible nitric oxide synthase in response to hypoxia in mouse embryonic fibroblast cells. Am J Physiol Cell Physiol 317:C31-C38 (2019). PubMed: 31067085
Suzuki Y et al. Aquaporin Positron Emission Tomography Differentiates Between Grade III and IV Human Astrocytoma. Neurosurgery 82:842-846 (2018). PubMed: 28645205

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Flow Cytometry

Sample

Human Cell (cervical cancer cells)

Permeabilization

Yes - PBA

Specification

cervical cancer cells

Preparation

Cell harvesting/tissue preparation method: HeLa cells were detached with standard trypsinization, washed with PBS, fixed and stored at -20°C until use. a
Sample buffer: PBA: 0,1% PBS tween 20 + 1% BSA

Fixation

4% PFA for 10 min and 70% Ethanol

Abcam user community

Verified customer

Submitted Feb 07 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Human embryonic kidney)

Permeabilization

Yes - 0.25% Triton X

Specification

Human embryonic kidney

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Fixative

Formaldehyde

Dr. Nils Lindstrom

Verified customer

Submitted Oct 07 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% Triton-X100 in PBS

Specification

HeLa

Fixative

Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 29 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (12)

Customer reviews and Q&As

Flow Cytometry abreview for Anti-Aquaporin 1 antibody [EPR11588(B)]

Immunohistochemistry (Frozen sections) abreview for Anti-Aquaporin 1 antibody [EPR11588(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Aquaporin 1 antibody [EPR11588(B)]