Recombinant Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11588(B)] to Aquaporin 1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Aquaporin 1 antibody [EPR11588(B)]
See all Aquaporin 1 primary antibodies -
Description
Rabbit monoclonal [EPR11588(B)] to Aquaporin 1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Aquaporin 1 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P29972 -
Positive control
- Human fetal kidney, fetal lung and fetal heart lysates; Human kidney and lung tissues. ICC/IF: HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11588(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
Tissue specificity
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal lung lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Formalin-fixed, paraffin-embedded human colon tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Formalin-fixed, paraffin-embedded human breast tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
Formalin-fixed, paraffin-embedded human lung tissue stained for Aquaporin 1 with ab168387 (1/250 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
References (12)
ab168387 has been referenced in 12 publications.
- Wu J et al. Gene Therapy for Glaucoma by Ciliary Body Aquaporin 1 Disruption Using CRISPR-Cas9. Mol Ther 28:820-829 (2020). PubMed: 31981492
- Zhang G et al. Based on Histogram Analysis: ADCaqp Derived from Ultra-high b-Value DWI could be a Non-invasive Specific Biomarker for Rectal Cancer Prognosis. Sci Rep 10:10158 (2020). PubMed: 32576929
- Nam SA et al. Graft immaturity and safety concerns in transplanted human kidney organoids. Exp Mol Med 51:1-13 (2019). PubMed: 31776328
- Serman Y et al. Emerging new role of NFAT5 in inducible nitric oxide synthase in response to hypoxia in mouse embryonic fibroblast cells. Am J Physiol Cell Physiol 317:C31-C38 (2019). PubMed: 31067085
- Suzuki Y et al. Aquaporin Positron Emission Tomography Differentiates Between Grade III and IV Human Astrocytoma. Neurosurgery 82:842-846 (2018). PubMed: 28645205