Recombinant
RabMAb

Recombinant Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free (ab232399)

Overview

  • Product name

    Anti-Aquaporin 1 antibody [EPR20325] - BSA and Azide free
    See all Aquaporin 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20325] to Aquaporin 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Aquaporin 1 aa 100-200. The exact sequence is proprietary.
    Database link: P29972

  • Positive control

    • IHC-P: Human pancreas tissue.
  • General notes

    Ab232399 is the carrier-free version of ab219055. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20325
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity

    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on proximal tubules of human kidney is observed [PMID: 14592814]. 

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Membranous staining on vessels of human cardiac muscle is observed [PMID: 14592814]. 

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged Aquaporin 1 expression construct or a GFP only control, labeling Aquaporin 1 with ab219055 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.

    Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged Aquaporin 1 expression construct.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 647) (ab150079) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Aquaporin 1 construct cells labeling Aquaporin 1 with ab219055 at 1/700 dilution (right). A rabbit monoclonal IgG (ab172730) was used as isotype control (left). Goat anti rabbit IgG (Alexa Fluor® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

    Quadrant markers were set based on control antibody staining (ab172730) and GFP expression. Only GFP positive population (Q2 of right panel) give Aquaporin 1 positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

  • Aquaporin 1 was immunoprecipitated from 0.35 mg of whole cell lysate from HEK-293T (Human epithelial cell line from embryonic kidney) transfected with a GFP-tagged human Auaporin 1 construct, with ab219055 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab219055 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct, 10 µg (Input).

    Lane 2: ab219055 IP in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219055 in whole cell lysate from HEK-293T transfected with a GFP-tagged human Auaporin 1 construct.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aquaporin 1 with ab219055 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on centroacinar cells of human pancreas is observed [PMID: 12801959].  Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219055).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab232399 has not yet been referenced specifically in any publications.

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