Anti-Aquaporin 1 antibody - N-terminal (ab189291)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Aquaporin 1 antibody - N-terminal
See all Aquaporin 1 primary antibodies -
Description
Rabbit polyclonal to Aquaporin 1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Rat Aquaporin 1 (N terminal).
Database link: P29975 -
Positive control
- Human kidney and pancreas tissues; rat kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab189291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | 1/200 - 1/400. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). It also detects glycosylated bands ~35-50 kDa. |
Target
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Function
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
Tissue specificity
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: Q02013 Mouse
- SwissProt: P29975 Rat
- Unigene: 76152 Human
see all -
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody - N-terminal (ab189291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling Aquaporin 1 using ab189291 at 10 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody - N-terminal (ab189291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Aquaporin 1 using ab189291 at 10 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody - N-terminal (ab189291)Immunofluorescence analysis of rat kidney tissue, labeling Aquaporin 1 using ab189291 at a 1/200 dilution (green). In blue DAPI staining.
Protocols
Datasheets and documents
References
ab189291 has not yet been referenced specifically in any publications.
