This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 29 kDa).
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Expressed in renal collecting tubules.
Involvement in disease
Diabetes insipidus, nephrogenic, autosomal
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
Water channel protein for renal collecting duct antibody
WCH CD antibody
Western blot - Anti-Aquaporin 2 antibody (ab110496)
All lanes : Anti-Aquaporin 2 antibody (ab110496) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203) Lane 2 : Kidney (Mouse) Tissue Lysate Lane 3 : Kidney (Rat) Tissue Lysate Lane 4 : Human kidney tissue lysate - total protein (ab30203) with Immunising peptide at 1 µg/ml Lane 5 : Kidney (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml Lane 6 : Kidney (Rat) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution