Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A 15 aa C terminal peptide from rat AQP2.

  • Positive control
    • Rat and human kidney cortex, apical membranes, inner and outer medulla (postranslational or glycosylation).
  • General notes
    In kidneys the AQP2 protein is mainly expressed in collecting ducts, which plays an important role in determining the final urinary output of water and solutes. The tissue section of this part will be a good positive control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 2 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in renal collecting tubules.
  • Involvement in disease
    Diabetes insipidus, nephrogenic, autosomal
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • Cellular localization
    Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Images

  • ab15081 staining Aquaporin 2 in rat kidney tissue section by IHC(P). Tissue underwent fixation in formalin, embedded in paraffin, heat mediated antigen retrieval in 10mM EDTA buffer pH 8.0 and blocking in 5% serum for 20 minutes at 20°C. The primary antibody, diluted 1/300 in TBSTween and incubated with sample for 1 hour at 20°C. A HRP conjugated goat polyclonal to rabbit was used undiluted as the secondary.  

    See Abreview

  • ab15081at a 1/250 dilution staining Aquaporin 2 in human kidney tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 20 minutes at 25°C. Heat mediated retrieval step performed using EDTA at 100°C for 20 minutes. Secondary used undiluted monoclonal Goat anti-rabbit, Leica Refine, HRP polymer.

    See Abreview

References

This product has been referenced in:
  • Airik R  et al. Renal-Retinal Ciliopathy Gene Sdccag8 Regulates DNA Damage Response Signaling. J Am Soc Nephrol N/A:N/A (2014). Human . Read more (PubMed: 24722439) »
  • Hoppensack A  et al. A human in vitro model that mimics the renal proximal tubule. Tissue Eng Part C Methods 20:599-609 (2014). IHC-P ; Human . Read more (PubMed: 24266327) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: unmasking solution from vector
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Yan Zhang

Verified customer

Submitted May 02 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy
Specification
Kidney
Blocking step
DAKO as blocking agent for 15 minute(s) · Concentration: 100%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 20 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney section - paraffin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - TritonX
Specification
Kidney section - paraffin
Blocking step
1%BSA, 10% Goat serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Archana Raman

Verified customer

Submitted May 26 2016

Answer

Thank you for your email. Please find below the tetsing discount code for ab85379. At the bottom of the email, I have listed the information regarding the free of charge replacement for ab15081 and ab58968.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab85379 in WB and rat/pig/sheep. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB and rat/pig/sheep and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

------------ FREE of charge REPLACEMENT ---------------------
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab15081 lot GR66676-1 and ab85379. You should receive both items tomorrow.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that the free of charge replacement vial for ab15081 is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply and the additional information. This is very helpful to us for our internal
I have checked our stock, but we have, unfortunately, only a new lot for ab15081 - which I'd be happy to send you free of charge. For ab7671 and ab58968 we have currently no new lot in stock.
But for alpha 1 Sodium Potassium ATPase, we have several other antibodies that have been tested in IHC-P and rat: ab2872, ab2867 and ab74945.
For Nephrin, we have no other antibody that has been tested in rat, but we have 3 antibodies that were tested in IHC-P with human samples:ab85379, ab82113 and ab92887.
I could send you one of these antibodies free of charge together with a testing discount for trying them on rat samples. For more information on our testing discount program please see below.
Alternatively, a credit or refund can be issued.
For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
Please let me know how you would like to proceed.
I look forward to hear back from you.

Read More

Answer

Thank you for contacting us.
I am sorry to hear that all 3 antibodies are not performing as expected.
Before proceeding with the free of charge replacements, could you please let me know the following experimental details:
1) What was the exact problem encountered (e.g. no signal, high background)?
2) What antibody dilution and incubation time was used?
3) What antigen retrieval step and blocking step were employed?
4) Is the detection system working well otherwise?
I look forward to hear back from you and resolve this problem.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Specification
Kidney
Fixative
10% NBF
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS/EDTA pH 9.0
Permeabilization
No
Blocking step
Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 0.25%

Ms. Elizabeth Chlipala

Verified customer

Submitted Aug 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, 100C, 20 minutes
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 02 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9,0
Permeabilization
No
Blocking step
Peroxidase Blocking solution (Dako) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM EDTA pH8.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 06 2008

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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