Overview

  • Product name

    Anti-Aquaporin 2 antibody [EPR21080]
    See all Aquaporin 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR21080] to Aquaporin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Aquaporin 2 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P41181

  • Positive control

    • WB: Human fetal kidney lysate; Mouse kidney lysate. IHC-P: Human, mouse and rat kidney tissues. IHC-Fr: Mouse and rat kidney tissues. IP: Aquaporin 2 IP in mouse kidney lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 28 kDa.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/3000.
IP 1/30.

Target

  • Function

    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity

    Expressed in renal collecting tubules.
  • Involvement in disease

    Diabetes insipidus, nephrogenic, autosomal
  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications

    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • Cellular localization

    Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of human kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • All lanes : Anti-Aquaporin 2 antibody [EPR21080] (ab199975) at 1/500 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 28 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds.

    We recommend customers optimize their lysis buffer and sample preparation for WB. This protein might aggregate during heating of samples.

    The molecular mass is consistent with literature (PMID 27405971; PMID 26109089).

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of mouse kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Aquaporin 2 with ab199975 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Luminal surface and tubular cytoplasmic staining on collecting duct of rat kidney is observed (PMID: 15703994, PMID: 19546380). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed mouse kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on mouse kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Nuclear counterstain: DAPI (blue).
    Permeabilization: 0.2% Triton X-100

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed rat kidney tissue labeling Aquaporin 2 (green) with ab199975 at 1/3000 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive luminal membrane staining and tubular cytoplasmic staining on rat kidney is observed (PMID: 9688853; 15703994; 19546380). Heat-mediated antigen retrieval was performed using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).

    Nuclear counterstain: DAPI (blue).
    Permeabilization: 0.2% Triton X-100

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary ab150077 at 1/1000 dilution.

  • Aquaporin 2 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab199975 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse kidney lysate 10 μg (input).
    Lane 2: ab199975 IP in mouse kidney lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199975 in mouse kidney lysate (-).

    Blocking/dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    We recommend customers optimize their lysis buffer and sample preparation for IP. This protein might aggregate during heating of samples.

    The molecular mass is consistent with the literature (PMID 27405971; PMID 26109089).

References

ab199975 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199975.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up