Anti-Aquaporin 3 antibody (ab125219)
Key features and details
- Rabbit polyclonal to Aquaporin 3
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Aquaporin 3 antibody
See all Aquaporin 3 primary antibodies -
Description
Rabbit polyclonal to Aquaporin 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra), ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
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Positive control
- WB: Mouse and rat kidney tissue lysates. HEK-293 and COS-7 whole cell lysates; IHC-P: Human lung cancer tissue. Mouse and rat kidney tissues; ICC: A431 cells; Flow Cyt (Intra).: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 32 kDa.
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IHC-P | (1) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use 1-3µg for 106 cells.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC |
Use a concentration of 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 32 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use 1-3µg for 106 cells. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC
Use a concentration of 2 µg/ml. |
Target
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Function
Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. -
Tissue specificity
Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Basolateral cell membrane. In collecting ducts of kidney. - Information by UniProt
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Database links
- Entrez Gene: 360 Human
- Entrez Gene: 11828 Mouse
- Entrez Gene: 65133 Rat
- Omim: 600170 Human
- SwissProt: Q92482 Human
- SwissProt: Q8R2N1 Mouse
- SwissProt: P47862 Rat
- Unigene: 234642 Human
see all -
Alternative names
- AQP 3 antibody
- AQP-3 antibody
- Aqp3 antibody
see all
Images
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All lanes : Anti-Aquaporin 3 antibody (ab125219) at 0.5 µg/ml (overnight at 4°C)
Lane 1 : Rat kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) for 1.5 hour at room temperature at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was then blocked with 5% Non-fat Milk / TBS for 1.5 hours at room temperature followed by incubation with ab125219. The membrane was then washed with TBS-0.1% Tween 3 times for 5 minutes each and probed with a Goat anti-rabbit IgG (HRP) secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labeling Aquaporin 3 with ab125219 at 1 μg/mL. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125219 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labeling Aquaporin 3 with ab125219 at 1 μg/mL. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125219 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling Aquaporin 3 with ab125219 at 1 μg/mL. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with ab125219 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Immunocytochemistry analysis of A431 (human epidermoid carcinoma cell line) cells labeling Aquaporin 3 with ab125219 at 2 μg/mL. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with ab125219 overnight at 4°C. DyLight® 488 Conjugated Goat Anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI and visualized using a fluorescence microscope with filter sets appropriate for the label used.
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Overlay histogram showing A431 (human epidermoid carcinoma cell line) cells stained with ab125219 (Blue line). The cells were blocked with 10% normal goat serum and then incubated with rabbit anti-Aquaporin 3 antibody (ab125219, 1μg/1x106 cells) for 30 min at 20°C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab125219 has been referenced in 49 publications.
- Cheng CC et al. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation. J Cell Physiol 237:1780-1789 (2022). PubMed: 34806177
- Zhao L et al. Changes in intestinal barrier protein expression and intestinal flora in a rat model of visceral hypersensitivity. Neurogastroenterol Motil 34:e14299 (2022). PubMed: 34821442
- Schmidt H et al. Serially passaged, conditionally reprogrammed nasal epithelial cells as a model to study epithelial functions and SARS-CoV-2 infection. Am J Physiol Cell Physiol 322:C591-C604 (2022). PubMed: 35196166
- Iwabuchi S et al. Immune Cells Profiles in the Different Sites of COVID-19-Affected Lung Lobes in a Single Patient. Front Med (Lausanne) 9:841170 (2022). PubMed: 35252273
- Montero P et al. Paclitaxel Induces Epidermal Molecular Changes and Produces Subclinical Alterations in the Skin of Gynecological Cancer Patients. Cancers (Basel) 14:N/A (2022). PubMed: 35267454