Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide (Rat) conjugated to KLH (C terminal).

  • Positive control
    • Rat kidney medulla or outer cortex.

Properties

Applications

Our Abpromise guarantee covers the use of ab15117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 3 peptide (ab80550).
ELISA 1/10000 - 1/50000.
IHC-Fr 1/50.

Target

  • Function
    Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
  • Tissue specificity
    Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Basolateral cell membrane. In collecting ducts of kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 3 antibody
    • AQP-3 antibody
    • Aqp3 antibody
    • AQP3_HUMAN antibody
    • Aquaglyceroporin-3 antibody
    • Aquaporin 3 (GIL blood group) antibody
    • Aquaporin 3 (Gill blood group) antibody
    • Aquaporin-3 antibody
    • Aquaporin3 antibody
    • GIL antibody
    • Gill blood group antibody
    see all

References

This product has been referenced in:
  • Ratovitski EA Phospho-?Np63a regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation. FEBS Lett 587:3581-6 (2013). Read more (PubMed: 24070899) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you very much for your interest in ab15117.

The C-terminal immunogen is predicted to be a cytoplasmic domain, not extracellular, based on information from the supplying lab. It may be suitable for FACS, but this application is untested. If you were to try it, we would recommend following an intracellular protocol:

https://www.abcam.com/index.html?pageconfig=resource&rid=11381

To our knowledge, ab15117 has not been tested in FACS. Therefore, I can offer a discount off a future purchase if you buy ab15117 now, test it inFACS and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab15117 in FACS. I will then send a discount code. This code must be issued before purchasing ab15117 so please wait for my reply before ordering.

2. Purchase ab15117 either by phone, fax, or online (www.abcam.com).

3. Test it in FACS.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15117 turns out to be unsuitable for FACS, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for submitting the Abreviews with ab9871, ab15117, ab110534 and ab18499 using dot blot. The results look really good.

I will be submitting your reviews manually. In order to issue the Abpoints generated to the relevant account, could you please confirm that you want them sent to your account (xxxxx).

I have issued instructions that the discount codes issued can be activated prior to the publishing of the reviews so you can now go ahead and use them to claim your free primary antibody.

Again thank you for providing us with these reviews. If you have any further queries please do not hesitate to contact us again.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 12 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Intestine)
Specification
Intestine
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Aug 29 2007

Answer

Thank you for your enquiry. This antibody has not been tested in these applications. We mistakenly had these applications on the datasheet but removed them on April 5, 2006. The antibody may work in these applications, but we have not tested it. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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