Anti-Aquaporin 4 antibody (ab125049)
Key features and details
- Rabbit polyclonal to Aquaporin 4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Aquaporin 4 antibody
See all Aquaporin 4 primary antibodies -
Description
Rabbit polyclonal to Aquaporin 4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
N-CTKGSYMEVEDNRSQVETED
, corresponding to C terminal amino acids 273-291 of Rat Aquaporin 4 (NP_036957.1). -
Positive control
- Rat kidney lysate; Rat kidney tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab125049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/400. | |
| WB | 1/1000 - 1/4000. Predicted molecular weight: 35 kDa. |
Target
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Function
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. -
Tissue specificity
Brain - muscle >> heart, kidney, lung, and trachea. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Post-translational
modificationsPhosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 361 Human
- Entrez Gene: 11829 Mouse
- Entrez Gene: 25293 Rat
- Omim: 600308 Human
- SwissProt: P55087 Human
- SwissProt: P55088 Mouse
- SwissProt: P47863 Rat
- Unigene: 315369 Human
see all -
Alternative names
- AQP 4 antibody
- AQP-4 antibody
- AQP4 antibody
see all
Images
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Western blot - Anti-Aquaporin 4 antibody (ab125049)All lanes : Anti-Aquaporin 4 antibody (ab125049) at 1/2000 dilution
All lanes : Rat kidney lysate
Predicted band size: 35 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 4 antibody (ab125049)ab125049, at 1/200 dilution, staining Aquaporin 4 in Rat kidney tissue by Immunofluorescence.
Protocols
References (0)
ab125049 has not yet been referenced specifically in any publications.
