Anti-Aquaporin 4 antibody (ab125049)

Rabbit polyclonal Aquaporin 4 antibody. Validated in WB, ICC/IF and tested in Mouse, Rat, Human. Immunogen corresponding to synthetic peptide.

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Overview

  • Product name

    Anti-Aquaporin 4 antibody
    See all Aquaporin 4 primary antibodies

  • Description

    Rabbit polyclonal to Aquaporin 4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide:

    N-CTKGSYMEVEDNRSQVETED

    , corresponding to C terminal amino acids 273-291 of Rat Aquaporin 4 (NP_036957.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Rat kidney lysate; Rat kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab125049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/400.
WB 1/1000 - 1/4000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

  • Tissue specificity

    Brain - muscle >> heart, kidney, lung, and trachea.

  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.

  • Domain

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

  • Post-translational
    modifications

    Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Images

  • Western blot - Anti-Aquaporin 4 antibody (ab125049)
    Western blot - Anti-Aquaporin 4 antibody (ab125049)
    All lanes : Anti-Aquaporin 4 antibody (ab125049) at 1/2000 dilution

    All lanes : Rat kidney lysate

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 4 antibody (ab125049)
    Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 4 antibody (ab125049)
    ab125049, at 1/200 dilution, staining Aquaporin 4 in Rat kidney tissue by Immunofluorescence.

References

ab125049 has not yet been referenced specifically in any publications.

Publishing research using ab125049? Please let us know so that we can cite the reference in this datasheet.

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