Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 4
  • Host species
    Rabbit
  • Specificity
    ab46182 is not suitable for staining Aquaporin 4 in IHC using paraffin embedded sections but some customers have successfully used the antibody in frozen sections (see submitted Abreviews). Please contact Scientific Support if you need further help.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-FrFl, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Sheep, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Aquaporin 4.

    Read Abcam's proprietary immunogen policy (Peptide available as ab46181.)

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Brain (Human), Heart (Human) and Brain (Rat).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Aquaporin 4 peptide (ab46181). Abcam recommends using milk as the blocking agent.
ICC/IF Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Function
    Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • Tissue specificity
    Brain - muscle >> heart, kidney, lung, and trachea.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications
    Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Images

  • All lanes : Anti-Aquaporin 4 antibody (ab46182) at 1 µg/ml (blocked with 3% milk)

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • ab46182 staining Aquaporin 4 in mouse RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunocytocchemical analysis of 33B cells originating from Rat nervous tissue oligodendroglioma, labelling AQP4 with ab46182 diluted 1/100. Cells were PFA fixed. Hematoxyling used to counterstained. Negative control given were ab46182 was not included.

    See Abreview

  • Immunohistochemical analysis of Pig brain tissue labelling Aquaporin 4 with ab46182 at 1/100. Tissue fixed by perfussion, antigen retrieval performed by microwaving in citric acid buffer. DAB staining. 

    See Abreview

References

This product has been referenced in:
  • Clayton AM  et al. Postpartum increases in cerebral edema and inflammation in response to placental ischemia during pregnancy. Brain Behav Immun 70:376-389 (2018). WB ; Rat . Read more (PubMed: 29588233) »
  • Farhat F  et al. Water deprivation decreases strength in fast twitch muscle in contrast to slow twitch muscle in rat. Acta Physiol (Oxf) 224:e13072 (2018). Read more (PubMed: 29633518) »
See all 15 Publications for this product

Customer reviews and Q&As

1-10 of 29 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - Tritonx100
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 01 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse adult brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
mouse adult brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 27 2017

Application
Immunohistochemistry free floating
Sample
Pig Tissue sections (Brain)
Specification
Brain

Audrey Lafrenaye

Verified customer

Submitted Jan 18 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Question
Answer

We recently compared this antibody against two other antibodies to Aquaporin 4 and they all gave a single band at about 46kDa. However a doublet at 46/48kDa is commonly seen when blotting for this target so if this is what you are seeing then I feel this data to be reasonable.

Read More

Answer

Thank you for your further questions.

I can confirm the following prices:
CD14 antibody [5A3B11B5] (ab36595) 100 ul £227.00
CD14 antibody [7] (ab49755) 200 ug £227.00
Delivery: £16.00 to United Kingdom

This information can be found on the individual online datasheets. Please ensure toselect the correct countryfrom thedropdown menu at the top right corner of the webpage. The correct price for the product and delivery charge in the UK will then show at the top of the page.

Regarding which of these antibodies is best for parafin embedded eye sections, I am sorry that neither of these CD14 antibodies have specifically been tested on eye sections as far as we are aware. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our 6 month guarantee in the applications and specieslisted on the datasheets. Therefore, any CD14 antibody tested in thespecies and application you are using should be suitable for your applications. Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

I hope this will be helpful. Should you have any further questions please do not hesitate to ask.

Read More

Answer

Thank you for your enquiry which has been forwarded to the scientific support team.

I believe our customer service team have already provided a quotation for you. I can confirm that prices are also available on the online datasheets.

Regarding the IHC application, I can confirm thatab46182is not tested in paraffin embedded sections, this would be indicated by 'IHC-P'.

I am pleased to let you know that Epitomics is now an Abcam company, and ab128906 5582-1 Anti-Aquaporin 4 antibody [EPR7040] is tested in IHC-P and in rat and human samples. so this is suitable and covered by our 6 month guarantee for IHC-P.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2a)
Loading amount
6 µg
Specification
Neuro2a
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 11 2012

1-10 of 29 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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