Immunocytochemistry/ Immunofluorescence abreview for Anti-Aquaporin 4 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Other product details

Dilution
1/100
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen Goat anti Rabbit AF555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000

Additional data

Additional Notes
Kelly Neuroblastoma cell line was cultured in RPMI/10% FCS on coverslips in 12 well plates.
Cells were washed with PBS and fixed in 4% PFA until staining.
Staining protocol:
Wash 2x2min PBS
Serum block - 10% serum 20mins at RT
Primary - add primary in PBS for 2h at RT
Wash 2x2min PBS
Secondary – Goat anti Rabbit AF555 (red) @ 1/1000 1h at RT
Wash 2x2min PBS
Counterstained with Hoechst 33258 (blue) for 10mins at RT.
Wash 3min in dH2O
Coverslips removed tapped dry and placed on glass slides and mounted in Invitrogen ProLong gold and kept at 4deg until imaged.

Abcam user community

Verified customer

Submitted Sep 26 2012

Sign up