Non-Abcam antibody was used: Invitrogen Goat anti Rabbit AF555 Host species: Goat Clonality: Polyclonal Conjugation: Alexa Fluor® 568
Kelly Neuroblastoma cell line was cultured in RPMI/10% FCS on coverslips in 12 well plates. Cells were washed with PBS and fixed in 4% PFA until staining. Staining protocol: Wash 2x2min PBS Serum block - 10% serum 20mins at RT Primary - add primary in PBS for 2h at RT Wash 2x2min PBS Secondary – Goat anti Rabbit AF555 (red) @ 1/1000 1h at RT Wash 2x2min PBS Counterstained with Hoechst 33258 (blue) for 10mins at RT. Wash 3min in dH2O Coverslips removed tapped dry and placed on glass slides and mounted in Invitrogen ProLong gold and kept at 4deg until imaged.
Abcam user community
Submitted Sep 26 2012
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