Immunohistochemistry (Frozen sections) abreview for Anti-Aquaporin 4 antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit AF488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
10um frozen section. Tissue fixed for 1h in 4%PFA, then 20% sucrose for 1h both at RT prior to embedding. Wash 3x3min in PBS. Block 10% goat serum for 30min at RT. Primary Ab diluted in PBS and incubated overnight at 4deg, also works by incubating for 2 hours at RT. Wash 3x3min in PBS. Secondary - goat anti-rabbit AF488 (green) 1/1000 in PBS for 1h at RT. Wash 3x3min in PBS Counterstained with Hoechst 33258 (blue) for 10mins at RT. Wash 3min in dH2O Mounted in Invitrogen ProLong gold and kept at 4deg until imaged.

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Submitted Apr 28 2014

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