Immunohistochemistry free floating abreview for Anti-Aquaporin 4 antibody

Good
Abreviews
Application
Immunohistochemistry free floating
Sample
Pig Tissue sections (Brain)
Specification
Brain

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NGS/PBS
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Biotinylated anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Tissue was 40um vibrotome brain tissue perfussion fixed with 4% para/0.2%glut and postfixed 1-3days. Used Microwave antigen retrieval (45 degrees 5min 2x in pH6 citric acid buffer) followed by 1% triton-X/5%NGS/PBS blocking/permeabilisation. Used Vectastain ABC kit to amplify the signal and DAB for visualization. Incubated tissue in DAB/H2O2 ~3min. Some general nuclei staining but also staining that looks like astrocytes.

Audrey Lafrenaye

Verified customer

Submitted Jan 18 2017

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