Tissue was 40um vibrotome brain tissue perfussion fixed with 4% para/0.2%glut and postfixed 1-3days. Used Microwave antigen retrieval (45 degrees 5min 2x in pH6 citric acid buffer) followed by 1% triton-X/5%NGS/PBS blocking/permeabilisation. Used Vectastain ABC kit to amplify the signal and DAB for visualization. Incubated tissue in DAB/H2O2 ~3min. Some general nuclei staining but also staining that looks like astrocytes.
Submitted Jan 18 2017
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