Western blot abreview for Anti-Aquaporin 4 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U87MG human glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
U87MG human glioblastoma cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 0.05% Tween
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 46 kDa Non-specific: 60 kDa

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 29 2018

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