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Hi Abcam, Please see below an email we have received from the same customer; I have tried the new lot that was sent, and while I did have some success (a faint band) with this antibody using a very high concentration (1/50) on rat brain tissue, I cannot get it to work on spinal cord tissue. Would it be possible to try a new antibody? The customer wishes to try AB41862 or AB81355, for WB using rat spinal cord tissue. They asked if you would recommend one over the other?
Asked on Feb 28 2012
Thank you for your enquiry.
I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Aquaporin 4 antibody tested in the species and application you are using should be suitable for your applications.
Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. I would recommend that when a user is deciding upon a product that they look for those products which have Abreviews and have been used in publications, if possible. When choosing an antibody to be used in Western blot or Flow Cytometry I would also recommend choosing a product which has either a peptide available or the peptide sequence published as the peptide can be used for blocking if necessary.
Please let me know which product your customer would prefer. I look forward to hearing from you. Should you have any further questions please do not hesitate to ask.
Thank you for contacting us.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 28 2012