Recombinant
RabMAb

Recombinant Anti-Aquaporin 4 antibody [EPR7040] (ab128906)

Overview

  • Product name

    Anti-Aquaporin 4 antibody [EPR7040]
    See all Aquaporin 4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7040] to Aquaporin 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Aquaporin 4 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P55087

  • Positive control

    • Human brain tissue.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 2.60 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7040
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • Tissue specificity

    Brain - muscle >> heart, kidney, lung, and trachea.
  • Sequence similarities

    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications

    Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Images

  • ab128906 at 1/100 dilution, staining Aquaporin 4 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Barrera V  et al. Neurovascular sequestration in paediatric P. falciparum malaria is visible clinically in the retina. Elife 7:N/A (2018). Read more (PubMed: 29578406) »
  • Salman MM  et al. Hypothermia increases aquaporin 4 (AQP4) plasma membrane abundance in human primary cortical astrocytes via a calcium/transient receptor potential vanilloid 4 (TRPV4)- and calmodulin-mediated mechanism. Eur J Neurosci 46:2542-2547 (2017). Read more (PubMed: 28925524) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
No
Specification
HeLa
Fixative
Methanol

Dr. Kirk Mcmanus

Verified customer

Submitted Sep 29 2016

Answer

Thank you for your further questions.

I can confirm the following prices:
CD14 antibody [5A3B11B5] (ab36595) 100 ul £227.00
CD14 antibody [7] (ab49755) 200 ug £227.00
Delivery: £16.00 to United Kingdom

This information can be found on the individual online datasheets. Please ensure toselect the correct countryfrom thedropdown menu at the top right corner of the webpage. The correct price for the product and delivery charge in the UK will then show at the top of the page.

Regarding which of these antibodies is best for parafin embedded eye sections, I am sorry that neither of these CD14 antibodies have specifically been tested on eye sections as far as we are aware. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our 6 month guarantee in the applications and specieslisted on the datasheets. Therefore, any CD14 antibody tested in thespecies and application you are using should be suitable for your applications. Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

I hope this will be helpful. Should you have any further questions please do not hesitate to ask.

Read More

Answer

Thank you for your enquiry which has been forwarded to the scientific support team.

I believe our customer service team have already provided a quotation for you. I can confirm that prices are also available on the online datasheets.

Regarding the IHC application, I can confirm thatab46182is not tested in paraffin embedded sections, this would be indicated by 'IHC-P'.

I am pleased to let you know that Epitomics is now an Abcam company, and ab128906 5582-1 Anti-Aquaporin 4 antibody [EPR7040] is tested in IHC-P and in rat and human samples. so this is suitable and covered by our 6 month guarantee for IHC-P.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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