Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog
  • Immunogen

    A 17 amino acid synthetic peptide corresponding to the carboxy terminus of rat Aquaporin 5

  • Positive control
    • Rat salivary gland lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab78486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 10 µg/ml.
ELISA Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 24 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 21704616

Target

  • Function
    Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
  • Involvement in disease
    Keratoderma, palmoplantar, Bothnian type
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Apical cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 5 antibody
    • AQP-5 antibody
    • AQP5 antibody
    • AQP5_HUMAN antibody
    • Aquaporin 5 antibody
    • Aquaporin-5 antibody
    see all

Images

  • Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/ml + Rat salivary gland tissue lysate

    Secondary
    Goat Anti-Rabbit IgG secondary antibody conjugated to HRP

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining Aquaporin 5 with ab78486 (1/2000) in rat lung. An anti-Rabbit secondary antibody and HRP-DAB was used. Tissues were processed using heat-induced epitope retrieval.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining Aquaporin 5 with ab78486 (1/500) in mouse trachea tissue. An anti-Rabbit secondary antibody and HRP-DAB was used. Tissues were processed using heat-induced epitope retrieval.

References

This product has been referenced in:
  • Xu Y  et al. Dietary nitrate protects submandibular gland from hyposalivation in ovariectomized rats via suppressing cell apoptosis. Biochem Biophys Res Commun 497:272-278 (2018). IHC-P . Read more (PubMed: 29432741) »
  • Xie L  et al. The Expression of AQP5 and UTs in the Sweat Glands of Uremic Patients. Biomed Res Int 2017:8629783 (2017). Read more (PubMed: 29279852) »

See all 18 Publications for this product

Customer reviews and Q&As

Application
CyTOF
Sample
Mouse Cell (Lung)
Specification
Lung
Username

Irit Milman

Verified customer

Submitted Nov 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

Submitted May 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (HEK cells transfected with AQP5-EGFP)
Permeabilization
Yes - 0,2% Saponin
Specification
HEK cells transfected with AQP5-EGFP
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 03 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primarly lung epithelia cells)
Specification
primarly lung epithelia cells
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Oct 08 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Enzymatic
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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