Overview

  • Product name
    Anti-Aquaporin 9 / AQP-9 antibody
    See all Aquaporin 9 / AQP-9 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 9 / AQP-9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Aquaporin 9/ AQP-9 (C terminal).
    (Peptide available as ab56306)

  • General notes

    Previously labelled as Aquaporin 9. 

Properties

Applications

Our Abpromise guarantee covers the use of ab15127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/10,000 - 1/50,000, or 0.5-1 ug/ml for affinity purified antibody. Control peptide can be used to coat ELISA plates at 1 ug/ml.
    WB: 1/1000 - 1/5000, or 1-10 ug/ml for affinity purified antibody using ECL. Predicted molecular weight: 35.4 kDa.
    Immunohistochemistry & Immunofluorescence: Neat serum can be used at 1:500 or more.

    Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.
    • Tissue specificity
      Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.
    • Sequence similarities
      Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • Domain
      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AQP 9 antibody
      • AQP-9 antibody
      • Aqp9 antibody
      • AQP9_HUMAN antibody
      • Aquaglyceroporin-9 antibody
      • Aquaporin 9 antibody
      • Aquaporin-9 antibody
      • Aquaporin9 antibody
      • HsT 17287 antibody
      • HsT17287 antibody
      • Small solute channel 1 antibody
      • SSC 1 antibody
      • SSC1 antibody
      see all

    References

    This product has been referenced in:
    • Han X  et al. Changes in the Expression of AQP4 and AQP9 in the Hippocampus Following Eclampsia-Like Seizure. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29351212) »
    • Sun Z  et al. Recombinant hirudin treatment modulates aquaporin-4 and aquaporin-9 expression after intracerebral hemorrhage in vivo. Mol Biol Rep 36:1119-27 (2009). WB, IF, IHC ; Rat . Read more (PubMed: 18574711) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    Thank you for your patience while I collected the information that you have requested. I apologize that this has taken so long.


    We currently have no matching products to the rat extracelluar domains for these targets that have been tested in flow. I have been gathering information about the immunogens to determine if some of our products may target the extracellular domain and may work in flow.



    AQP9: Our products ab15127 and ab15129 may recognize extracelluar site. The immunogens are from the C-terminal cytoplasmic domain of rat AQP9, not the extracellular domain.

    ECAD/CAD1: Ab53226 may/or may not recognize correct site but I have been unable to attain the information needed to determine whether the epitope is extracellular. I will let you know when I have that information. This product would qualify for our 100% Abreview Testing Discount.

    EpCAM: Product number ab68892 is rat compatable and recognizes an extracellular region but has not been tested in flow. This product would qualify for our 100% Abreview Testing Discount.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    This antibody recognizes Aquaporin 9. There is no significant sequence homology to any other AQPs. Species Crossreactivity: Human, mouse and rat. Species Sequence Homology: Mouse: 94%; human: 72%. For a polyclonal =70% is usually good enough to assume crossreactivity; but it has not been explicitly tested.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up