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Dear Technical Assistant, currently, we are starting with magnetic cell separation and we intend to use indirect magnetic labeling for the isolation ofepithelial cellsofrat. We are looking for primary antibodies againstcell surface moleculesuitable forflow cytometry (FC)(the cells must remain alive to be cultured after sorting). Please, could you clarify some features of the antibodies of your portfolio listed below? 1. Aquaporin 9 Is there any antibody able to recognize the extracellular domain of rat AQP9? Is it suitable for FC? 2.E-cadherin Does the item #5296 cross-react with rat? Is it applied for FC? Please, could you recommend us another primary antibody able to recognize an extracellular domain of rat E-cadherin suitable for FC? 3. Which antibody is able to recognize anextracellular domain of rat Epcam and could be applied for FC?
Asked on Feb 08 2012
Thank you for contacting us.
Thank you for your patience while I collected the information that you have requested. I apologize that this has taken so long.
We currently have no matching products to the rat extracelluar domains for these targets that have been tested in flow. I have been gathering information about the immunogens to determine if some of our products may target the extracellular domain and may work in flow.
AQP9: Our products ab15127 and ab15129 may recognize extracelluar site. The immunogens are from the C-terminal cytoplasmic domain of rat AQP9, not the extracellular domain.
ECAD/CAD1: Ab53226 may/or may not recognize correct site but I have been unable to attain the information needed to determine whether the epitope is extracellular. I will let you know when I have that information. This product would qualify for our 100% Abreview Testing Discount.
EpCAM: Product number ab68892 is rat compatable and recognizes an extracellular region but has not been tested in flow. This product would qualify for our 100% Abreview Testing Discount.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 09 2012