Overview

  • Product name

    Aqueous Mounting Medium
    See all mounting media reagents
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details
  • General notes

    Aqueous mounting medium for use in fluorescence microscopy. Enhances FITC intensity and reduces fluorescence fading. Ready-to-use. 

    Reagent: 6 ml

    Other products for IHC

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Procedure:

    FOR USE IN FLUORESCENCE MICROSCOPY

    After completion of immunostaining, remove slide from water, apply sufficient drops of Aqueous Mounting Medium upon section and mount coverglass. Coverslip should be immobilized within 30 minutes at room temperature. To avoid air bubbles within mounting medium, DO NOT SHAKE VIAL. If air bubbles are evident during coverslipping, carefully trim the vial tip with a sharp blade to create a larger opening and improve media flow. These directions are recommendations; however, they may be altered according to procedures established in-house.

    IHC Protocol Summary

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab128982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

References

This product has been referenced in:

  • Shaimerdenova M  et al. The effects of antiviral treatment on breast cancer cell line. Infect Agent Cancer 12:18 (2017). Read more (PubMed: 28344640) »
  • Dong C  et al. Ketamine Affects the Neurogenesis of Rat Fetal Neural Stem Progenitor Cells via the PI3K/Akt-p27 Signaling Pathway. Birth Defects Res B Dev Reprod Toxicol 101:355-63 (2014). Read more (PubMed: 25231110) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

nice item, fast shipping

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This mounting medium has very good quality. It does not cause bubbles if you are very careful and only use a small drop. My slides are still good several months after using the mounting medium.

Abcam user community

Verified customer

Submitted Nov 23 2016

Answer

My pleasure as always. If I can be of any further help, please do not hesitate to ask.

Until then, have a lovely week.

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Answer

Thank you for contacting us and your interest in our products.

For the experiment the customer is planning to perform, the BrightMount (ab103746) or BrightMount/Plus (ab103748) would be most appropriate and would be compatible with the fluorescent dyes the customer wants to use. The BrightMount/Plus provides the added advantage of containing an anti-fading agent which preserves strong fluorescent staining, allowing the user to view slides months after they have been stained.

I hope this information has been of help. If you have any further questions, please do not hesitate to let me know.

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Answer

Thank you for contacting us.


The alternatives are as follows

1. ab103746; https://www.abcam.com/BrightMount-25ml-Aqueous-Mounting-Medium-for-Fluorescent-Staining-ab103746.html

or ab128982; https://www.abcam.com/Aqueous-Mounting-Medium-ab128982.html

2. We do not sell Poly L Lysine




5. ab133412; https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
6. We have similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
7. Similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
8. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

9. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

10. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

11. We do not sell SAP

12. We don have clone 4A6 antibody. We however have ab32; https://www.abcam.com/c-Myc-antibody-9E10-ChIP-Grade-ab32.htmlwhich is better than clone 4A6.

13. We do not sell protein purification kit Ni-NTA column Cat.No.31014 (50 columns)

14. We do not sell protein purification kit protein purification kit Ni-NTA slurry Cat. No.30250

15. We do not sell NEB chitin-intein Tag cat # E6901S

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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