Aqueous Mounting Medium (ab128982)

Reviews (2)Q&A (3)References (7)

Overview

  • Product name

    Aqueous Mounting Medium
    See all mounting media reagents

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details

  • General notes

    Aqueous mounting medium for use in fluorescence microscopy. Enhances FITC intensity and reduces fluorescence fading. Ready-to-use. 

    Reagent: 6 ml

    Other products for IHC

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Procedure:

    FOR USE IN FLUORESCENCE MICROSCOPY

    After completion of immunostaining, remove slide from water, apply sufficient drops of Aqueous Mounting Medium upon section and mount coverglass. Coverslip should be immobilized within 30 minutes at room temperature. To avoid air bubbles within mounting medium, DO NOT SHAKE VIAL. If air bubbles are evident during coverslipping, carefully trim the vial tip with a sharp blade to create a larger opening and improve media flow. These directions are recommendations; however, they may be altered according to procedures established in-house.

    IHC Protocol Summary

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (7)

Publishing research using ab128982? Please let us know so that we can cite the reference in this datasheet.

ab128982 has been referenced in 7 publications.

  • Franco-Barraza J  et al. Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices. Methods Cell Biol 156:109-160 (2020). PubMed: 32222216
  • Kwok SS  et al. Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts. J Clin Med 9:N/A (2020). PubMed: 33171906
  • Wei K  et al. Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice. Neuroscience 415:59-69 (2019). PubMed: 31301366
  • Sun X  et al. (-)-Epigallocatechin-3-gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. Oncol Rep 42:425-435 (2019). PubMed: 31180522
  • Shaalan A & Proctor G Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury. Sci Rep 9:11339 (2019). PubMed: 31383943
  • Shaimerdenova M  et al. The effects of antiviral treatment on breast cancer cell line. Infect Agent Cancer 12:18 (2017). PubMed: 28344640
  • Dong C  et al. Ketamine Affects the Neurogenesis of Rat Fetal Neural Stem Progenitor Cells via the PI3K/Akt-p27 Signaling Pathway. Birth Defects Res B Dev Reprod Toxicol 101:355-63 (2014). PubMed: 25231110

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Aqueous Mounting Medium

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
The medium is very good to cover immune stainings. It is easy to dose and use. The preparations are clear and easy to microscopy.

Abcam user community

Verified customer

Submitted Aug 29 2019

nice item, fast shipping

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
This mounting medium has very good quality. It does not cause bubbles if you are very careful and only use a small drop. My slides are still good several months after using the mounting medium.

Abcam user community

Verified customer

Submitted Nov 23 2016

Question

Hi,

Thank you very much for this information, I will forward to the customer. Your customer service is excellent! Thank you.

Regards

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Answer

My pleasure as always. If I can be of any further help, please do not hesitate to ask.

Until then, have a lovely week.

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Question

Dear Support Group,

One of our customers has the following question:

1. Aqueous Mounting Medium (ab128982)
2. BrightMount 25ml (Aqueous Mounting Medium for Fluorescent Staining) (ab103746)
3. BrightMount/Plus 25ml Aqueous Mounting Medium (Anti-fading) for Fluorescent Staining (ab103748)

Which would be the best to use for IF. I am staining cells on a coverslip with AlexaFluor 488 and 660.

Please advise.
Thank you for your help.

Best regards

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Answer

Thank you for contacting us and your interest in our products.

For the experiment the customer is planning to perform, the BrightMount (ab103746) or BrightMount/Plus (ab103748) would be most appropriate and would be compatible with the fluorescent dyes the customer wants to use. The BrightMount/Plus provides the added advantage of containing an anti-fading agent which preserves strong fluorescent staining, allowing the user to view slides months after they have been stained.

I hope this information has been of help. If you have any further questions, please do not hesitate to let me know.

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Question

Please let us have best alternate products for the above at your earliest.
1. Fluoromount Mounting medium F4680-25ML

2. Poly L Lysine cat # P8920

100G Gelbes blutlaugensalz

rotes blutlaugensalz 702587 250G

5. Hybond ECL (30 cm × 3m) 0.45um Nitrocellulose Membrane cat RPN303D

6. Amersham Hyperfilm ECL (18 x 24 cm) 50 sheets Cat # 28-9068-36

7. Amersham ECL Plus Western Blotting Detection System (18 x24 cm) 50 sheets

8. Pepstatin Cat # 20037 5MG

9. Aprotinin 11388 10 MG

10. Leupeptin 18413 5 MG

11. SAP 78390 1000 UN

12. Anti-Myc Tag, cat # clone 4A6 200

13. protein purification kit Ni-NTA column Cat.No.31014 (50 columns)

14. protein purification kit Ni-NTA slurry Cat. No.30250

15. NEB chitin-intein Tag cat # E6901S
Please update at an early time.
Sincere regards,

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Answer

Thank you for contacting us.


The alternatives are as follows

1. ab103746; https://www.abcam.com/BrightMount-25ml-Aqueous-Mounting-Medium-for-Fluorescent-Staining-ab103746.html

or ab128982; https://www.abcam.com/Aqueous-Mounting-Medium-ab128982.html

2. We do not sell Poly L Lysine




5. ab133412; https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
6. We have similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
7. Similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
8. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

9. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

10. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

11. We do not sell SAP

12. We don have clone 4A6 antibody. We however have ab32; https://www.abcam.com/c-Myc-antibody-9E10-ChIP-Grade-ab32.htmlwhich is better than clone 4A6.

13. We do not sell protein purification kit Ni-NTA column Cat.No.31014 (50 columns)

14. We do not sell protein purification kit protein purification kit Ni-NTA slurry Cat. No.30250

15. We do not sell NEB chitin-intein Tag cat # E6901S

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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