Overview

  • Product name
  • Description
    Rabbit polyclonal to ARA9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide: MADLIARLRE DGIQKRVIQE GRGELPDFQD GTKATFHFRT LHSDNEGSVI, corresponding to a region within N terminal amino acids 1-50 of Mouse ARA9

  • Positive control
    • NIH3T3 lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20709796
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting.
    Cellular negative regulator of the hepatitis B virus (HBV) X protein.
  • Tissue specificity
    Widely expressed. Higher levels seen in the heart, placenta and skeletal muscle. Not expressed in the liver.
  • Involvement in disease
    Defects in AIP are a cause of familial isolated pituitary adenoma (FIPA) [MIM:102200].
    Defects in AIP are a cause of growth hormone-secreting pituitary adenoma (GHSPA) [MIM:102200]; also known as familial isolated somatotropinomas (FIS) or isolated familial somatotropinoma (IFS) or familial somatotrophinoma or acromegaly due to pituitary adenoma.
    Defects in AIP are a cause of ACTH-secreting pituitary adenoma (ASPA) [MIM:219090]; also known as pituitary Cushing disease. A pituary adenoma resulting in excessive production of adrenocorticotropic hormone. This leads to hypersecretion of cortisol by the adrenal glands and ACTH-dependent Cushing syndrome. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes.
    Defects in AIP are a cause of prolactin-secreting pituitary adenoma (PSPA) [MIM:600634]; also known as prolactinoma. Prolactin-secreting pituitary adenoma is the most common type of hormonally active pituitary adenoma.
  • Sequence similarities
    Contains 1 PPIase FKBP-type domain.
    Contains 2 TPR repeats.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AH receptor interacting protein antibody
    • AH receptor-interacting protein antibody
    • AIP antibody
    • AIP_HUMAN antibody
    • ARA 9 antibody
    • ARA9 antibody
    • Aryl hydrocarbon receptor interacting protein antibody
    • Aryl-hydrocarbon receptor-interacting protein antibody
    • fa03h10 antibody
    • FKBP 16 antibody
    • FKBP 37 antibody
    • FKBP16 antibody
    • FKBP37 antibody
    • HBV X associated protein 2 antibody
    • HBV X associated protein antibody
    • HBV X-associated protein 2 antibody
    • HBVX associated protein antibody
    • Immunophilin homolog ARA 9 antibody
    • Immunophilin homolog ARA9 antibody
    • SMTPHN antibody
    • XAP 2 antibody
    • XAP-2 antibody
    • XAP2 antibody
    see all

Images

  • Anti-ARA9 antibody (ab48833) at 1.25 µg/ml + NIH3T3 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 40 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Raitila A  et al. Mice with Inactivation of Aryl Hydrocarbon Receptor-Interacting Protein (Aip) Display Complete Penetrance of Pituitary Adenomas with Aberrant ARNT Expression. Am J Pathol 177:1969-76 (2010). IHC-P ; Mouse . Read more (PubMed: 20709796) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for your call today and for letting us know about the trouble with ab48833. I apologize for the inconsistent results using this antibody. As we discussed, I am sending a free of charge vial of ab115588 on the order ***, which I expect to arrive around Thursday January 12. Please keep me updated about the results using this antibody, and let me know if you have any questions or if there is anything else that we can do for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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