Key features and details
- Rabbit polyclonal to ARAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARAP1 antibody
DescriptionRabbit polyclonal to ARAP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide, corresponding to a region between amino acids 1160-1210 of Human ARAP1 (NP_056057.1).
- HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 136 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPhosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
Tissue specificityDetected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
Sequence similaritiesContains 1 Arf-GAP domain.
Contains 4 PH domains.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
- Information by UniProt
- 2410002L19Rik antibody
- ANK repeat and PH domain-containing protein 1 antibody
- ARAP 1 antibody
All lanes : Anti-ARAP1 antibody (ab99382) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 136 kDa
Exposure time: 30 seconds
Detection of ARAP1 by Western Blot of Immunprecipitate.
ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab99382 has not yet been referenced specifically in any publications.