Overview

  • Product name
  • Description
    Goat polyclonal to ARAP3
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CTSSPPSSQPLT

    , corresponding to C terminal amino acids 1533-1544 of Human ARAP3.

  • General notes
    GenBank Accession Number – NP_071926

Properties

Applications

Our Abpromise guarantee covers the use of ab5911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 21076469
IP Use at an assay dependent dilution. PubMed: 21076469
WB Use at an assay dependent dilution. Can be blocked with Human ARAP3 peptide (ab23118). PubMed: 21076469

Target

  • Relevance
    The ARAP3 gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape.
  • Database links
  • Alternative names
    • Ankyrin repeat and plekstrin homology domains containing protein 3 antibody
    • ARAP 3 antibody
    • ARF GAP antibody
    • ARF-GAP RHO-GAP ankyrin repeat and plekstrin homology domains containing protein 3 antibody
    • CENTD3 antibody
    • FLJ21065 antibody
    • Phosphoinositide binding protein antibody
    • PtdIns 3 4 5 P3 binding protein antibody
    • RHO GAP antibody
    see all

Images

  • All lanes : Anti-ARAP3 antibody (ab5911) at 1/500 dilution

    Lane 1 : HEK293 negative control
    Lane 2 : HEK293 overexpressed GFP-ARAP3
    Lane 3 : Whole tissue lysate prepared from mouse heart
    Lane 4 : Whole tissue lysate prepared from mouse brain
    Lane 5 : Whole tissue lysate prepared from mouse diaphragma
    Lane 6 : Whole tissue lysate prepared from mouse fundus
    Lane 7 : Whole tissue lysate prepared from rabbit heart

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : Alexa-Fluor 680 conjugated donkey polyclonal at 1/10000 dilution


    Expected band for HEK overexpression 200kDa.
    Expected band for rabbit heart 170kDa.

    See Abreview

References

This product has been referenced in:
  • Yagi R  et al. ARAP3 inhibits peritoneal dissemination of scirrhous gastric carcinoma cells by regulating cell adhesion and invasion. Oncogene : (2010). WB, IHC-P, IP ; Human . Read more (PubMed: 21076469) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (HEART. BRAIN,DIAPHRAGMA,FUNDUS)
Loading amount
100 µg
Specification
HEART. BRAIN,DIAPHRAGMA,FUNDUS
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Oddysey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 02 2012

Answer

Thank you for your call today and for your questions. I will let you know once I have more information about the homology of the ab67278 immunogen to the murine protein. Please see below for the discount codes to test ab121972 and ab5911 in mouse, and please let me know if you have any questions or if there is anything else that I can do for you.

DISCOUNT CODES:

Ab121972: ***
Ab5911: ***

Expiration date: July 15, 2012

These codes will each give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for each antibody in mouse samples,and include the code in the “Additional Comments” section of the corresponding Abreview. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Eachcode will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your enquiry. Thus far, we have not received any feedback concerning this antibody. All the information that we currently have is on the online datasheet. Ab5911 is a Fast Track antibody: Fast-Track antibodies are immunogen affinity purified and are shown by ELISA to have high titre values against their immunogen. We are currently testing them for use in Western blotting. At this stage, there can be delays. We sometimes experience problems in searching for / developing the correct lysate or knock-out cell-line. In addition, certain products can be difficult to characterise, due, for instance, to low expression levels or a lack of expression in easily available lysates. We realise that these products might be very suitable for your research and do not wish to delay this; therefore, we release them early with the following understanding: As Fast-Track antibodies are not yet fully characterised we are unable to offer our normal level of technical support. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and therefore we are unable to offer a refund if the antibody does not work in your application. However, Fast-Track antibodies are offered at a reduced price. We would greatly appreciate your feedback on these antibodies, whether positive or negative. We will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. If you have any more questions, please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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