Product nameAnti-ARAP3 antibody
See all ARAP3 primary antibodies
DescriptionGoat polyclonal to ARAP3
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
General notesGenBank Accession Number – NP_071926
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab5911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution. PubMed: 21076469|
|IP||Use at an assay dependent dilution. PubMed: 21076469|
|WB||Use at an assay dependent dilution. Can be blocked with Human ARAP3 peptide (ab23118). PubMed: 21076469|
RelevanceThe ARAP3 gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape.
- Ankyrin repeat and plekstrin homology domains containing protein 3 antibody
- ARAP 3 antibody
- ARF GAP antibody
All lanes : Anti-ARAP3 antibody (ab5911) at 1/500 dilution
Lane 1 : HEK293 negative control
Lane 2 : HEK293 overexpressed GFP-ARAP3
Lane 3 : Whole tissue lysate prepared from mouse heart
Lane 4 : Whole tissue lysate prepared from mouse brain
Lane 5 : Whole tissue lysate prepared from mouse diaphragma
Lane 6 : Whole tissue lysate prepared from mouse fundus
Lane 7 : Whole tissue lysate prepared from rabbit heart
Lysates/proteins at 100 µg per lane.
All lanes : Alexa-Fluor 680 conjugated donkey polyclonal at 1/10000 dilution
Expected band for HEK overexpression 200kDa.Expected band for rabbit heart 170kDa.