Anti-Arc antibody (ab203056)
Key features and details
- Rabbit polyclonal to Arc
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Arc antibody
See all Arc primary antibodies -
Description
Rabbit polyclonal to Arc -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human Arc conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q7LC44 -
Positive control
- Flow Cyt (Intra): A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
Notes |
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Flow Cyt (Intra)
1/20 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
Target
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Function
Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. -
Sequence similarities
Belongs to the ARC/ARG3.1 family. -
Cellular localization
Cytoplasm > cytoskeleton. Endosome. Cytoplasmic vesicle > secretory vesicle > acrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 23237 Human
- Entrez Gene: 54323 Rat
- Omim: 612461 Human
- SwissProt: Q7LC44 Human
- SwissProt: Q63053 Rat
- Unigene: 40888 Human
- Unigene: 741621 Human
- Unigene: 10086 Rat
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Alternative names
- activity regulated cytoskeleton associated protein antibody
- activity regulated gene 3.1 protein homolog antibody
- Activity-regulated cytoskeleton-associated protein antibody
see all
Images
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A549 cells probed with ab203056at 1/100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H L) Alexa Fluor 488 Conjugated Secondary.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling ARC with ab203056 at 1/200 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, PE conjugated at 1:200 for 40 minutes at 37°C. DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab203056 has been referenced in 1 publication.
- Wang N et al. Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathway. Cell Physiol Biochem 44:1696-1714 (2017). PubMed: 29216639