Anti-Arc antibody (ab203056)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Arc antibody
See all Arc primary antibodies -
Description
Rabbit polyclonal to Arc -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Arc conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q7LC44 -
Positive control
- Rat brain tissue, A549 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
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| WB | 1/100 - 1/1000. Predicted molecular weight: 45 kDa. | |
| Flow Cyt | 1/20 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. -
Sequence similarities
Belongs to the ARC/ARG3.1 family. -
Cellular localization
Cytoplasm > cytoskeleton. Endosome. Cytoplasmic vesicle > secretory vesicle > acrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 23237 Human
- Entrez Gene: 11838 Mouse
- Entrez Gene: 54323 Rat
- Omim: 612461 Human
- SwissProt: Q7LC44 Human
- SwissProt: Q9WV31 Mouse
- SwissProt: Q63053 Rat
- Unigene: 40888 Human
see all -
Alternative names
- activity regulated cytoskeleton associated protein antibody
- activity regulated gene 3.1 protein homolog antibody
- Activity-regulated cytoskeleton-associated protein antibody
see all
Images
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Flow Cytometry - Anti-Arc antibody (ab203056)A549 cells probed with ab203056 at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H L) Alexa Fluor 488 Conjugated Secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody (ab203056)Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling ARC with ab203056 at 1/200 dilution, followed by secondary antibody Goat Anti-Rabbit IgG, PE conjugated at 1:200 for 40 minutes at 37°C. DAPI (5ug/ml, blue) was used to stain the cell nuclei.
Datasheets and documents
References
ab203056 has not yet been referenced specifically in any publications.
