Anti-Arc antibody (ab203056)

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Key features and details

  • Rabbit polyclonal to Arc
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Arc antibody
    See all Arc primary antibodies

  • Description

    Rabbit polyclonal to Arc

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Arc conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q7LC44

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab203056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.
Flow Cyt 1/20 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.

  • Sequence similarities

    Belongs to the ARC/ARG3.1 family.

  • Cellular localization

    Cytoplasm > cytoskeleton. Endosome. Cytoplasmic vesicle > secretory vesicle > acrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • activity regulated cytoskeleton associated protein antibody
    • activity regulated gene 3.1 protein homolog antibody
    • Activity-regulated cytoskeleton-associated protein antibody
    • Activity-regulated gene 3.1 protein homolog antibody
    • Arc antibody
    • ARC/ARG3.1 antibody
    • ARC_HUMAN antibody
    • Arg 3.1 antibody
    • Arg3.1 antibody
    see all

Images

  • Flow Cytometry - Anti-Arc antibody (ab203056)
    Flow Cytometry - Anti-Arc antibody (ab203056)

    A549 cells probed with ab203056 at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H L) Alexa Fluor 488 Conjugated Secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody (ab203056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody (ab203056)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling ARC with ab203056 at 1/200 dilution, followed by  secondary antibody  Goat Anti-Rabbit IgG, PE conjugated at 1:200 for 40 minutes at 37°C. DAPI (5ug/ml, blue) was used to stain the cell nuclei.

References (1)

Publishing research using ab203056? Please let us know so that we can cite the reference in this datasheet.

ab203056 has been referenced in 1 publication.

  • Wang N  et al. Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathway. Cell Physiol Biochem 44:1696-1714 (2017). PubMed: 29216639

Customer reviews and Q&As

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Immunohistochemistry (Frozen sections) abreview for Anti-Arc antibody

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (brain (coronal, through Area X and striatum)))
Permeabilization
Yes - 0.3% Triton X
Specification
brain (coronal, through Area X and striatum))
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Sandeep Kumar

Verified customer

Submitted Jun 30 2020

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