Recombinant
RabMAb

Recombinant Anti-Arc antibody [EPR18950] (ab183183)

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Overview

  • Product name

    Anti-Arc antibody [EPR18950]
    See all Arc primary antibodies

  • Description

    Rabbit monoclonal [EPR18950] to Arc

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse Arc aa 1-250. The exact sequence is proprietary.
    Database link: Q9WV31

  • Positive control

    • WB: Human hippocampus lysate; Mouse and rat hippocampus and brain lysates; Neuro-2a and SH-SY5Y cell lysates. IHC-P: Mouse hippocampus and cerebral cortex tissues. IP: Mouse brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab183183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/30.

Target

  • Function

    Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.

  • Sequence similarities

    Belongs to the ARC/ARG3.1 family.

  • Cellular localization

    Cytoplasm > cytoskeleton. Endosome. Cytoplasmic vesicle > secretory vesicle > acrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • activity regulated cytoskeleton associated protein antibody
    • activity regulated gene 3.1 protein homolog antibody
    • Activity-regulated cytoskeleton-associated protein antibody
    • Activity-regulated gene 3.1 protein homolog antibody
    • Arc antibody
    • ARC/ARG3.1 antibody
    • ARC_HUMAN antibody
    • Arg 3.1 antibody
    • Arg3.1 antibody
    see all

Images

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    Lanes 1-4 : Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution
    Lane 5 : Anti-Arc antibody [EPR18950] (ab183183) at 1/5000 dilution

    Lane 1 : Mouse hippocampus lysate at 20 µg
    Lane 2 : Rat hippocampus lysate at 20 µg
    Lane 3 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
    Lane 5 : Human hippocampus lysate at 10 µg

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    All lanes : Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat liver lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sparse nuclear staining (arrows) in the dentate gyrus of mouse hippocampus (PMID: 19750198, PMID: 19628007). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on neurons of mouse cerebral cortex. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183)
    Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183)

    Arc was immunoprecipitated from 0.35 mg of mouse brain lysate with ab183183 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183183 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab183183 IP in mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183183 in mouse brain lysate.

    Exposure time: 1 second

    Blocking/dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Zhong Y  et al. Brain-derived neurotrophic factor inhibits hyperglycemia-induced apoptosis and downregulation of synaptic plasticity-related proteins in hippocampal neurons via the PI3K/Akt pathway. Int J Mol Med 43:294-304 (2019). Read more (PubMed: 30365051) »
  • Zhang Z  et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 30297870) »
See all 2 Publications for this product

Publishing research using ab183183? Please let us know so that we can cite the reference in this datasheet.

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