Recombinant Anti-Arc antibody [EPR18950] (ab183183)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18950] to Arc
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Arc antibody [EPR18950]
See all Arc primary antibodies -
Description
Rabbit monoclonal [EPR18950] to Arc -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P MouseIP MouseWB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human hippocampus lysate; Mouse and rat hippocampus and brain lysates; Neuro-2a and SH-SY5Y cell lysates. IHC-P: Mouse hippocampus and cerebral cortex tissues. IP: Mouse brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18950 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC |
Mouse
|
IHC-Fr |
Mouse
Rat
|
IHC-P |
Mouse
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IP |
Mouse
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WB |
Mouse
Rat
Human
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Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/30. |
Target
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Function
Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. -
Sequence similarities
Belongs to the ARC/ARG3.1 family. -
Cellular localization
Cytoplasm > cytoskeleton. Endosome. Cytoplasmic vesicle > secretory vesicle > acrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 23237 Human
- Entrez Gene: 11838 Mouse
- Entrez Gene: 54323 Rat
- Omim: 612461 Human
- SwissProt: Q7LC44 Human
- SwissProt: Q9WV31 Mouse
- SwissProt: Q63053 Rat
- Unigene: 40888 Human
see all -
Alternative names
- activity regulated cytoskeleton associated protein antibody
- activity regulated gene 3.1 protein homolog antibody
- Activity-regulated cytoskeleton-associated protein antibody
see all
Images
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Lanes 1-4 : Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution
Lane 5 : Anti-Arc antibody [EPR18950] (ab183183) at 1/5000 dilution
Lane 1 : Mouse hippocampus lysate at 20 µg
Lane 2 : Rat hippocampus lysate at 20 µg
Lane 3 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
Lane 5 : Human hippocampus lysate at 10 µg
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat liver lysate
Lane 8 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sparse nuclear staining (arrows) in the dentate gyrus of mouse hippocampus (PMID: 19750198, PMID: 19628007). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on neurons of mouse cerebral cortex. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Arc was immunoprecipitated from 0.35 mg of mouse brain lysate with ab183183 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183183 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 µg (Input).
Lane 2: ab183183 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183183 in mouse brain lysate.
Exposure time: 1 second
Blocking/dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (3)
ab183183 has been referenced in 3 publications.
- Privitera L et al. Experience Recruits MSK1 to Expand the Dynamic Range of Synapses and Enhance Cognition. J Neurosci 40:4644-4660 (2020). PubMed: 32376781
- Zhong Y et al. Brain-derived neurotrophic factor inhibits hyperglycemia-induced apoptosis and downregulation of synaptic plasticity-related proteins in hippocampal neurons via the PI3K/Akt pathway. Int J Mol Med 43:294-304 (2019). PubMed: 30365051
- Zhang Z et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . PubMed: 30297870