Overview

  • Product name

    Anti-ARD1A antibody [3G3B9]
    See all ARD1A primary antibodies
  • Description

    Mouse monoclonal [3G3B9] to ARD1A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
  • Immunogen

    Recombinant fragment corresponding to Human ARD1A aa 111-235. Expressed in E. coli.
    Database link: P41227

  • Positive control

    • Flow Cytometry: SMMC-7721 cells. ICC/IF: HeLa cells. WB: COS-7, HEK-293, HL-60, MCF7, HeLa, NIH/3T3, and C2C12 cell lysates; Recombinant human ARD1A (amino acids 111-235) protein; Human ARD1A (amino acids 111-235)-hIgGFc transfected HEK-293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab234076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 26 kDa.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the acetyltransferase family. ARD1 subfamily.
    Contains 1 N-acetyltransferase domain.
  • Post-translational
    modifications

    Cleaved by caspases during apoptosis.
  • Cellular localization

    Cytoplasm. Nucleus. According to PubMed:12464182 it is cytoplasmic. According to PubMed:15496142, it is nuclear and cytoplasmic. Also present in the free cytosolic and cytoskeleton-bound polysomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha N acetyltransferase 1A antibody
    • ARD1 antibody
    • ARD1 homolog N acetyltransferase (S. cerevisiae) antibody
    • ARD1 homolog A N acetyltransferase (S. cerevisiae) antibody
    • ARD1 homolog A N acetyltransferase antibody
    • ARD1A antibody
    • DXS707 antibody
    • MGC71248 antibody
    • N acetyltransferase ARD1, human homolog of antibody
    • N alpha acetyltransferase 10 NatA catalytic subunit antibody
    • N terminal acetyltransferase complex ARD1 subunit homolog A antibody
    • N(alpha) acetyltransferase 10 NatA catalytic subunit antibody
    • N-alpha-acetyltransferase 10 antibody
    • N-terminal acetyltransferase complex ARD1 subunit homolog A antibody
    • Naa10 antibody
    • NAA10_HUMAN antibody
    • NatA catalytic subunit antibody
    • TE2 antibody
    see all

Images

  • All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 6 : NIH-3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 7 : C2C12 (mouse myoblast cell line) cell lysate

    Predicted band size: 26 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ARD1A (green) using ab234076 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AlexaFluor®555-Phalloidin. 

  • Flow Cytometry analysis of SMMC-7721 (human hepatocarcinoma cell line) cells, labeling ARD1A with ab234076 at a 1/200 dilution (green) compared to a negative control (red).

  • All lanes : Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Human ARD1A (amino acids 111-235)-hIgGFc transfected HEH-293 cell lysate

    Predicted band size: 26 kDa

  • Anti-ARD1A antibody [3G3B9] (ab234076) at 1/100 dilution + Recombinant human ARD1A (amino acids 111-235) protein

    Predicted band size: 26 kDa



    Expected recombinant MW is 47.2 kDa

References

ab234076 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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