Overview

  • Product name

    Anti-ARF GAP 2 antibody [EPR8497]
    See all ARF GAP 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8497] to ARF GAP 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARF GAP 2 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, Human skeletal muscle, HepG2, Human fetal heart and HeLa whole cell lysate (ab150035); Human colon tissue and breast carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
    • Sequence similarities

      Contains 1 Arf-GAP domain.
    • Cellular localization

      Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADP-ribosylation factor GTPase-activating protein 2 antibody
      • ARF GAP 2 antibody
      • ARFG2_HUMAN antibody
      • ARFGAP2 antibody
      • FLJ14576 antibody
      • FLJ26000 antibody
      • GTPase activating protein ZNF289 antibody
      • GTPase-activating protein ZNF289 antibody
      • IRZ antibody
      • Nbla10535 antibody
      • Zfp289 antibody
      • Zinc finger protein 289 antibody
      • Zinc finger protein 289 ID1 regulated antibody
      • ZNF289 antibody
      see all

    Images

    • All lanes : Anti-ARF GAP 2 antibody [EPR8497] (ab133768) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Human fetal heart lysate
      Lane 5 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 56 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133768 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133768.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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