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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)

  • Datasheet
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Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
  • Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
  • Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8304(B)] to ARF1 + ARF3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-ARF1 + ARF3 antibody [EPR8304(B)]
    See all ARF1+ARF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8304(B)] to ARF1 + ARF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARF3 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2, Raji cell lysates; Human fetal brain lysate; Human stomach tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8304(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HepG2 cytoplasmic extract lysate (ab14659)

Applications

Our Abpromise guarantee covers the use of ab129003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Cellular localization

      ARF1: Golgi apparatus. Cytoplasm > perinuclear region. ARF3: Golgi apparatus. Cytoplasm > perinuclear region.
    • Database links

      • Entrez Gene: 375 Human
      • Entrez Gene: 377 Human
      • Entrez Gene: 11840 Mouse
      • Entrez Gene: 11842 Mouse
      • Entrez Gene: 140940 Rat
      • Entrez Gene: 64310 Rat
      • Omim: 103180 Human
      • Omim: 103190 Human
      • SwissProt: P61204 Human
      • SwissProt: P84077 Human
      • SwissProt: P61205 Mouse
      • SwissProt: P84078 Mouse
      • SwissProt: P61206 Rat
      • SwissProt: P84079 Rat
      • Unigene: 119177 Human
      • Unigene: 286221 Human
      • Unigene: 221298 Mouse
      • Unigene: 371546 Mouse
      • Unigene: 6836 Mouse
      • Unigene: 106440 Rat
      • Unigene: 93735 Rat
      see all

    Images

    • Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/20000 dilution

      Lane 1 : Full length human ARF-1 recombinant protein
      Lane 2 : Full length human ARF-3 recombinant protein

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 20 kDa
      Observed band size: 23 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST

      Human ARF-1 recombinant protein, (Cat#:ab123538 )
      Human ARF-3 recombinant protein, (Cat#:ab113581 )

    • Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa
      Observed band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)

      ab129003, at 1/50 dilution, staining ARF3 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab129003? Please let us know so that we can cite the reference in this datasheet.

    ab129003 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab129003.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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