Recombinant Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8304(B)] to ARF1 + ARF3
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-ARF1 + ARF3 antibody [EPR8304(B)]
See all ARF1+ARF3 primary antibodies -
Description
Rabbit monoclonal [EPR8304(B)] to ARF1 + ARF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ARF3 aa 100-200. The exact sequence is proprietary.
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Positive control
- HeLa, HepG2, Raji cell lysates; Human fetal brain lysate; Human stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8304(B) -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
ARF1: Golgi apparatus. Cytoplasm > perinuclear region. ARF3: Golgi apparatus. Cytoplasm > perinuclear region. -
Database links
- Entrez Gene: 375 Human
- Entrez Gene: 377 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 11842 Mouse
- Entrez Gene: 140940 Rat
- Entrez Gene: 64310 Rat
- Omim: 103180 Human
- Omim: 103190 Human
see all
Images
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All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/20000 dilution
Lane 1 : Full length human ARF-1 recombinant protein
Lane 2 : Full length human ARF-3 recombinant protein
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
Human ARF-1 recombinant protein, (Cat#:ab123538 )
Human ARF-3 recombinant protein, (Cat#:ab113581 ) -
All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
ab129003, at 1/50 dilution, staining ARF3 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab129003 has been referenced in 2 publications.
- Carr D et al. Multiple roles for Bcl-3 in mammary gland branching, stromal collagen invasion, involution and tumor pathology. Breast Cancer Res 24:40 (2022). PubMed: 35681213
- Chaouat AE et al. The HHV-6A Proteins U20 and U21 Target NKG2D Ligands to Escape Immune Recognition. Front Immunol 12:714799 (2021). PubMed: 34721381