Recombinant
RabMAb

Recombinant Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)

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Overview

  • Product name

    Anti-ARF1 + ARF3 antibody [EPR8304(B)]
    See all ARF1+ARF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8304(B)] to ARF1 + ARF3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ARF3 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2, Raji cell lysates; Human fetal brain lysate; Human stomach tissue.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    Images

    • Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/20000 dilution

      Lane 1 : Full length human ARF-1 recombinant protein
      Lane 2 : Full length human ARF-3 recombinant protein

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 20 kDa
      Observed band size: 23 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST

      Human ARF-1 recombinant protein, (Cat#:ab123538 )
      Human ARF-3 recombinant protein, (Cat#:ab113581 )

    • Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Western blot - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa
      Observed band size: 20 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003)

      ab129003, at 1/50 dilution, staining ARF3 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab129003 has not yet been referenced specifically in any publications.

    Publishing research using ab129003? Please let us know so that we can cite the reference in this datasheet.

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