Product nameAnti-ARF1 antibody
See all ARF1 primary antibodies
DescriptionRabbit polyclonal to ARF1
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Mouse, Dog, Human
Predicted to work with: Rat, Cow
- HeLa, U2 OS, 3T3, NRK and MDCK cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol, 69% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|IP||Use at 4 µg/mg of lysate.|
|WB||1/1000 - 1/2000. Predicted molecular weight: 21 kDa.|
FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
- Information by UniProt
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
All lanes : Anti-ARF1 antibody (ab183576) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U2 OS cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : NRK cell lysate
Lane 5 : MDCK cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : goat anti-rabbit IgG-HRP at 1/1500 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Detection of ARF1 in Immunoprecipitates of HeLa cell lysate. Antigen-antibody complexes were formed by incubating 500μg of HeLa whole cell lysate with 2μg ab183576 (lane 2) compared with HeLa cell lysate as a positive control (lane 1). For detection, ab183576 was used at 1/1000 dilution.
Immunofluorescent analysis of HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling ARF1 with ab183576 at 1/100 dilution followed with DyLight 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.
ab183576 has not yet been referenced specifically in any publications.