Anti-ARF1 antibody (ab58578)
Key features and details
- Goat polyclonal to ARF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARF1 antibody
See all ARF1 primary antibodies -
Description
Goat polyclonal to ARF1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
EGLDWLSNQLRNQK
by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1 -
General notes
This antibody is expected to recognize all four reported isoforms.
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Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
This antibody is expected to recognize all four reported isoforms. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Xenopus laevis
Cynomolgus monkey
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 375 Human
- Entrez Gene: 380166 Xenopus laevis
- Omim: 103180 Human
- SwissProt: Q4R5P2 Cynomolgus monkey
- SwissProt: P84077 Human
- SwissProt: P51643 Xenopus laevis
- Unigene: 286221 Human
- Unigene: 3045 Xenopus laevis
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Alternative names
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
Images
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Western blot - Anti-ARF1 antibody (ab58578)Anti-ARF1 antibody (ab58578) at 1 µg/ml + HepG2 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab58578 has been referenced in 8 publications.
- Steiner B et al. ER remodeling by the large GTPase atlastin promotes vacuolar growth of Legionella pneumophila. EMBO Rep 18:1817-1836 (2017). PubMed: 28835546
- Escoll P et al. Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages. Cell Host Microbe 22:302-316.e7 (2017). PubMed: 28867389
- Humphreys D et al. Inhibition of WAVE Regulatory Complex Activation by a Bacterial Virulence Effector Counteracts Pathogen Phagocytosis. Cell Rep 17:697-707 (2016). PubMed: 27732847
- Hoffmann C et al. Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Cell Microbiol 16:1034-52 (2014). PubMed: 24373249
- Humphreys D et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). PubMed: 24085844
- Koronakis V et al. WAVE regulatory complex activation by cooperating GTPases Arf and Rac1. Proc Natl Acad Sci U S A 108:14449-54 (2011). WB . PubMed: 21844371
- Sharma P et al. Endoplasmic reticulum protein targeting of phospholamban: a common role for an N-terminal di-arginine motif in ER retention? PLoS One 5:e11496 (2010). WB ; Human . PubMed: 20634894
- Khan AP et al. Quantitative proteomic profiling of prostate cancer reveals a role for miR-128 in prostate cancer. Mol Cell Proteomics 9:298-312 (2010). WB ; Human . PubMed: 19955085