Anti-ARF1 antibody (ab58578)
- Datasheet
- References (6)
- Protocols
Overview
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Product nameAnti-ARF1 antibody
See all ARF1 primary antibodies -
DescriptionGoat polyclonal to ARF1
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Host speciesGoat
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human
Predicted to work with: Xenopus laevis, Cynomolgus monkey
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Immunogen
Synthetic peptide:
EGLDWLSNQLRNQK
by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1 -
Positive control
- Recombinant Human ARF1 protein (ab123538) can be used as a positive control in WB. HEK293, A431 and HepG2 cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Primary antibody notesThis antibody is expected to recognize all four reported isoforms.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab58578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa). | |
| ELISA | 1/8000. |
Target
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FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
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Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
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Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
- Information by UniProt
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Database links
- Entrez Gene: 338058 Cow
- Entrez Gene: 100856517 Dog
- Entrez Gene: 375 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 64310 Rat
- Entrez Gene: 380166 Xenopus laevis
- Omim: 103180 Human
- SwissProt: P84080 Cow
see all -
Alternative names
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
Images
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Western blot - Anti-ARF1 antibody (ab58578)Anti-ARF1 antibody (ab58578) at 1 µg/ml + HepG2 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Datasheets and documents
Protocols
References
This product has been referenced in:
- Humphreys D et al. Inhibition of WAVE Regulatory Complex Activation by a Bacterial Virulence Effector Counteracts Pathogen Phagocytosis. Cell Rep 17:697-707 (2016). Read more (PubMed: 27732847) »
- Hoffmann C et al. Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Cell Microbiol 16:1034-52 (2014). Read more (PubMed: 24373249) »
