Anti-ARF1 antibody (ab58578)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-ARF1 antibody
See all ARF1 primary antibodies -
Description
Goat polyclonal to ARF1 -
Host species
Goat -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Dog, Human
Predicted to work with: Xenopus laevis, Cynomolgus monkey
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Immunogen
Synthetic peptide:
EGLDWLSNQLRNQK
by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1 -
Positive control
- Recombinant Human ARF1 protein (ab123538) can be used as a positive control in WB. HEK293, A431 and HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
This antibody is expected to recognize all four reported isoforms. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab58578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa). | |
| ELISA | 1/8000. |
Target
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Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 338058 Cow
- Entrez Gene: 100856517 Dog
- Entrez Gene: 375 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 64310 Rat
- Entrez Gene: 380166 Xenopus laevis
- Omim: 103180 Human
- SwissProt: P84080 Cow
see all -
Alternative names
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
Images
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Western blot - Anti-ARF1 antibody (ab58578)Anti-ARF1 antibody (ab58578) at 1 µg/ml + HepG2 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Steiner B et al. ER remodeling by the large GTPase atlastin promotes vacuolar growth of Legionella pneumophila. EMBO Rep 18:1817-1836 (2017). Read more (PubMed: 28835546) »
- Escoll P et al. Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages. Cell Host Microbe 22:302-316.e7 (2017). Read more (PubMed: 28867389) »
