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    arf1-antibody-ab58578.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-ARF1 antibody (ab58578)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (8)

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Western blot - Anti-ARF1 antibody (ab58578)

    Key features and details

    • Goat polyclonal to ARF1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant Human ARF1 protein (Tagged) (ab235860)

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    Overview

    • Product name

      Anti-ARF1 antibody
      See all ARF1 primary antibodies
    • Description

      Goat polyclonal to ARF1
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      EGLDWLSNQLRNQK

      by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes


      This antibody is expected to recognize all four reported isoforms.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Primary antibody notes

      This antibody is expected to recognize all four reported isoforms.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation
      • Signal Transduction
      • Protein Trafficking
      • ER Proteins

    Associated products

    • Assay kits

      • Arf1 Activation Assay Kit (ab211170)
    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant Human ARF1 protein (Tagged) (ab235860)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab58578 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Xenopus laevis
    Cynomolgus monkey
    Application Abreviews Notes
    WB (4)
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Arf family.
    • Cellular localization

      Golgi apparatus. Cytoplasm > perinuclear region.
    • Target information above from: UniProt accession P84077 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 375 Human
      • Entrez Gene: 380166 Xenopus laevis
      • Omim: 103180 Human
      • SwissProt: Q4R5P2 Cynomolgus monkey
      • SwissProt: P84077 Human
      • SwissProt: P51643 Xenopus laevis
      • Unigene: 286221 Human
      • Unigene: 3045 Xenopus laevis
      • Alternative names

        • ADP Ribosylation Factor 1 antibody
        • ADP-ribosylation factor 1 antibody
        • ARF 1 antibody
        • ARF1 antibody
        • ARF1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-ARF1 antibody (ab58578)
        Western blot - Anti-ARF1 antibody (ab58578)
        Anti-ARF1 antibody (ab58578) at 1 µg/ml + HepG2 cell lysate at 35 µg

        Developed using the ECL technique.

        Predicted band size: 21 kDa
        Observed band size: 18 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab58578? Please let us know so that we can cite the reference in this datasheet.

      ab58578 has been referenced in 8 publications.

      • Steiner B  et al. ER remodeling by the large GTPase atlastin promotes vacuolar growth of Legionella pneumophila. EMBO Rep 18:1817-1836 (2017). PubMed: 28835546
      • Escoll P  et al. Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages. Cell Host Microbe 22:302-316.e7 (2017). PubMed: 28867389
      • Humphreys D  et al. Inhibition of WAVE Regulatory Complex Activation by a Bacterial Virulence Effector Counteracts Pathogen Phagocytosis. Cell Rep 17:697-707 (2016). PubMed: 27732847
      • Hoffmann C  et al. Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Cell Microbiol 16:1034-52 (2014). PubMed: 24373249
      • Humphreys D  et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). PubMed: 24085844
      • Koronakis V  et al. WAVE regulatory complex activation by cooperating GTPases Arf and Rac1. Proc Natl Acad Sci U S A 108:14449-54 (2011). WB . PubMed: 21844371
      • Sharma P  et al. Endoplasmic reticulum protein targeting of phospholamban: a common role for an N-terminal di-arginine motif in ER retention? PLoS One 5:e11496 (2010). WB ; Human . PubMed: 20634894
      • Khan AP  et al. Quantitative proteomic profiling of prostate cancer reveals a role for miR-128 in prostate cancer. Mol Cell Proteomics 9:298-312 (2010). WB ; Human . PubMed: 19955085

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-ARF1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Dog Cell lysate - whole cell (MDCK)
      Loading amount
      15 µg
      Specification
      MDCK
      Gel Running Conditions
      Reduced Denaturing (15% tris-glycine)
      Blocking step
      Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 19 2012

      Western blot abreview for Anti-ARF1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Cow Cell lysate - whole cell (Bovine kidney cells)
      Loading amount
      15 µg
      Specification
      Bovine kidney cells
      Gel Running Conditions
      Reduced Denaturing (15 % SDS-PAGE)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 06 2012

      Western blot abreview for Anti-ARF1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Recombinant protein (Human recombinant Arf-1 expressed in E.coli)
      Loading amount
      10 µg
      Specification
      Human recombinant Arf-1 expressed in E.coli
      Gel Running Conditions
      Reduced Denaturing (10% Tris-glycine)
      Blocking step
      Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 30 2012

      Western blot abreview for Anti-ARF1 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Xenopus laevis Cell lysate - other (Xenopus laevis egg extract)
      Loading amount
      5 µg
      Specification
      Xenopus laevis egg extract
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
      Read More

      Dr. Anne-Lore Schlaitz

      Verified customer

      Submitted Jan 28 2009

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