Anti-ARF1 antibody (ab76082)
Key features and details
- Rabbit polyclonal to ARF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ARF1 antibody
See all ARF1 primary antibodies -
Description
Rabbit polyclonal to ARF1 -
Host species
Rabbit -
Specificity
ab76082 does not cross react to other members of the Ras family or to other ARF members. This antibody will label ARF 1 isoform 1, 2, 3 and 4. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to a unique epitope on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desired antigenicity.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Predicted molecular weight: 21 kDa.
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Notes |
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WB
1/500. Predicted molecular weight: 21 kDa. |
Target
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Function
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
Sequence similarities
Belongs to the small GTPase superfamily. Arf family. -
Cellular localization
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 375 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 64310 Rat
- Omim: 103180 Human
- SwissProt: P84077 Human
- SwissProt: P84078 Mouse
- SwissProt: P84079 Rat
- Unigene: 286221 Human
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Alternative names
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab76082 has not yet been referenced specifically in any publications.